Next Article in Journal
Recent Perspectives on Sex Differences in Compulsion-Like and Binge Alcohol Drinking
Previous Article in Journal
Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation
Open AccessArticle

Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus

1
Department of Microbiology, Graduate School, Daejeon University, Daejeon 34520, Korea
2
Soonchunhyang Institute of Medi-Bio Science, Soonchunhyang University, Cheonan 31151, Korea
3
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA
4
Department of Systems Biotechnology, Konkuk University, Seoul 143-701, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Stephen Bustin
Int. J. Mol. Sci. 2021, 22(7), 3777; https://doi.org/10.3390/ijms22073777
Received: 16 February 2021 / Revised: 4 April 2021 / Accepted: 5 April 2021 / Published: 6 April 2021
(This article belongs to the Section Molecular Microbiology)
The APSES family proteins are transcription factors (TFs) with a basic helix-loop-helix domain, known to regulate growth, development, secondary metabolism, and other biological processes in Aspergillus species. In the genome of the human opportunistic pathogenic fungus Aspergillus fumigatus, five genes predicted to encode APSES TFs are present. Here, we report the characterization of one of these genes, called mbsA (Afu7g05620). The deletion (Δ) of mbsA resulted in significantly decreased hyphal growth and asexual sporulation (conidiation), and lowered mRNA levels of the key conidiation genes abaA, brlA, and wetA. Moreover, ΔmbsA resulted in reduced spore germination rates, elevated sensitivity toward Nikkomycin Z, and significantly lowered transcripts levels of genes associated with chitin synthesis. The mbsA deletion also resulted in significantly reduced levels of proteins and transcripts of genes associated with the SakA MAP kinase pathway. Importantly, the cell wall hydrophobicity and architecture of the ΔmbsA asexual spores (conidia) were altered, notably lacking the rodlet layer on the surface of the ΔmbsA conidium. Comparative transcriptomic analyses revealed that the ΔmbsA mutant showed higher mRNA levels of gliotoxin (GT) biosynthetic genes, which was corroborated by elevated levels of GT production in the mutant. While the ΔmbsA mutant produced higher amount of GT, ΔmbsA strains showed reduced virulence in the murine model, likely due to the defective spore integrity. In summary, the putative APSES TF MbsA plays a multiple role in governing growth, development, spore wall architecture, GT production, and virulence, which may be associated with the attenuated SakA signaling pathway. View Full-Text
Keywords: APSES transcription factor; Aspergillus fumigatus; MbsA; rodlet layer; gliotoxin; virulence; transcriptomics APSES transcription factor; Aspergillus fumigatus; MbsA; rodlet layer; gliotoxin; virulence; transcriptomics
Show Figures

Figure 1

MDPI and ACS Style

Choi, Y.-H.; Jun, S.-C.; Lee, M.-W.; Yu, J.-H.; Shin, K.-S. Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus. Int. J. Mol. Sci. 2021, 22, 3777. https://doi.org/10.3390/ijms22073777

AMA Style

Choi Y-H, Jun S-C, Lee M-W, Yu J-H, Shin K-S. Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus. International Journal of Molecular Sciences. 2021; 22(7):3777. https://doi.org/10.3390/ijms22073777

Chicago/Turabian Style

Choi, Yong-Ho; Jun, Sang-Cheol; Lee, Min-Woo; Yu, Jae-Hyuk; Shin, Kwang-Soo. 2021. "Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus" Int. J. Mol. Sci. 22, no. 7: 3777. https://doi.org/10.3390/ijms22073777

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop