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Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids
Article

Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes

1
BIOTARGEN, UNICAEN, Normandie University, 14000 Caen, France
2
Dielen Laboratory, 50110 Tourlaville, France
3
Normandy Center for Genomic and Personalized Medicine, Department of Genetics, Caen University Hospital, 14000 Caen, France
4
Service de Chirurgie Orthopédique, Clinique Saint-Martin, 14000 Caen, France
5
CNRS UMR 5305, Laboratory of Tissue Biology and Therapeutic Engineering, University Claude Bernard Lyon 1, Univ Lyon, 69367 Lyon, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Seung-Hong Lee
Int. J. Mol. Sci. 2021, 22(7), 3693; https://doi.org/10.3390/ijms22073693
Received: 24 February 2021 / Revised: 26 March 2021 / Accepted: 28 March 2021 / Published: 1 April 2021
Cartilage is a non-innervated and non-vascularized tissue. It is composed of one main cell type, the chondrocyte, which governs homeostasis within the cartilage tissue, but has low metabolic activity. Articular cartilage undergoes substantial stresses that lead to chondral defects, and inevitably osteoarthritis (OA) due to the low intrinsic repair capacity of cartilage. OA remains an incurable degenerative disease. In this context, several dietary supplements have shown promising results, notably in the relief of OA symptoms. In this study, we investigated the effects of collagen hydrolysates derived from fish skin (Promerim®30 and Promerim®60) and fish cartilage (Promerim®40) on the phenotype and metabolism of human articular chondrocytes (HACs). First, we demonstrated the safety of Promerim® hydrolysates on HACs cultured in monolayers. Then we showed that, Promerim® hydrolysates can increase the HAC viability and proliferation, while decreasing HAC SA-β-galactosidase activity. To evaluate the effect of Promerim® on a more relevant model of culture, HAC were cultured as organoids in the presence of Promerim® hydrolysates with or without IL-1β to mimic an OA environment. In such conditions, Promerim® hydrolysates led to a decrease in the transcript levels of some proteases that play a major role in the development of OA, such as Htra1 and metalloproteinase-1. Promerim® hydrolysates downregulated HtrA1 protein expression. In contrast, the treatment of cartilage organoids with Promerim® hydrolysates increased the neosynthesis of type I collagen (Promerim®30, 40 and 60) and type II collagen isoforms (Promerim®30 and 40), the latter being the major characteristic component of the cartilage extracellular matrix. Altogether, our results demonstrate that the use of Promerim® hydrolysates hold promise as complementary dietary supplements in combination with the current classical treatments or as a preventive therapy to delay the occurrence of OA in humans. View Full-Text
Keywords: human chondrocytes; matrix-associated autologous chondrocyte implantation (MACI); osteoarthritis; collagen; collagen hydrolysates; interleukin-1; catabolic markers; senescence; in vitro repair human chondrocytes; matrix-associated autologous chondrocyte implantation (MACI); osteoarthritis; collagen; collagen hydrolysates; interleukin-1; catabolic markers; senescence; in vitro repair
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MDPI and ACS Style

Bourdon, B.; Cassé, F.; Gruchy, N.; Cambier, P.; Leclercq, S.; Oddoux, S.; Noël, A.; Lafont, J.E.; Contentin, R.; Galéra, P. Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes. Int. J. Mol. Sci. 2021, 22, 3693. https://doi.org/10.3390/ijms22073693

AMA Style

Bourdon B, Cassé F, Gruchy N, Cambier P, Leclercq S, Oddoux S, Noël A, Lafont JE, Contentin R, Galéra P. Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes. International Journal of Molecular Sciences. 2021; 22(7):3693. https://doi.org/10.3390/ijms22073693

Chicago/Turabian Style

Bourdon, Bastien; Cassé, Frédéric; Gruchy, Nicolas; Cambier, Pierre; Leclercq, Sylvain; Oddoux, Sarah; Noël, Antoine; Lafont, Jérôme E.; Contentin, Romain; Galéra, Philippe. 2021. "Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes" Int. J. Mol. Sci. 22, no. 7: 3693. https://doi.org/10.3390/ijms22073693

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