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Article

Pseudo-Starvation Driven Energy Expenditure Negatively Affects Ovarian Follicle Development

1
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2
Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands
3
German Institute of Human Nutrition in Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany
4
Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Satoshi Kishigami
Int. J. Mol. Sci. 2021, 22(7), 3557; https://doi.org/10.3390/ijms22073557
Received: 26 February 2021 / Revised: 23 March 2021 / Accepted: 27 March 2021 / Published: 30 March 2021
(This article belongs to the Section Molecular Endocrinology and Metabolism)
In the present investigation, we examined whether a change in whole body energy fluxes could affect ovarian follicular development, employing mice ectopically expressing uncoupling protein 1 in skeletal muscle (UCP1-TG). Female UCP1-TG and wild-type (WT) mice were dissected at the age of 12 weeks. Energy intake and expenditure, activity, body weight and length, and body composition were measured. Plasma insulin, glucose, leptin, plasma fibroblast growth factor 21 (FGF21) and plasma insulin-like growth factor 1 (IGF1) levels were analyzed and ovarian follicle and corpus luteum numbers were counted. IGF1 signaling was analyzed by immunohistochemical staining for the activation of insulin receptor substrate 1/2 (IRS1/2) and AKT. UCP1-TG female mice had increased energy expenditure, reduced body size, maintained adiposity, and decreased IGF1 concentrations compared to their WT littermates, while preantral and antral follicle numbers were reduced by 40% and 60%, respectively. Corpora lutea were absent in 40% of the ovaries of UCP1-TG mice. Phospho-IRS1, phospho-AKT -Ser473 and -Thr308 immunostaining was present in the granulosa cells of antral follicles in WT ovaries, but faint to absent in the antral follicles of UCP1-TG mice. In conclusion, the reduction in circulating IGF1 levels due to the ectopic expression of UCP1 is associated with reduced immunostaining of the IRS1-PI3/AKT pathway, which may negatively affect ovarian follicle development and ovulation. View Full-Text
Keywords: ovarian follicular development; IGF1; energy metabolism; UCP1; IRS1; AKT ovarian follicular development; IGF1; energy metabolism; UCP1; IRS1; AKT
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MDPI and ACS Style

Meng, L.; Coleman, V.; Zhao, Y.; Ost, M.; Voigt, A.; Bunschoten, A.; Keijer, J.; Teerds, K.; Klaus, S. Pseudo-Starvation Driven Energy Expenditure Negatively Affects Ovarian Follicle Development. Int. J. Mol. Sci. 2021, 22, 3557. https://doi.org/10.3390/ijms22073557

AMA Style

Meng L, Coleman V, Zhao Y, Ost M, Voigt A, Bunschoten A, Keijer J, Teerds K, Klaus S. Pseudo-Starvation Driven Energy Expenditure Negatively Affects Ovarian Follicle Development. International Journal of Molecular Sciences. 2021; 22(7):3557. https://doi.org/10.3390/ijms22073557

Chicago/Turabian Style

Meng, Li, Verena Coleman, Yu Zhao, Mario Ost, Anja Voigt, Annelies Bunschoten, Jaap Keijer, Katja Teerds, and Susanne Klaus. 2021. "Pseudo-Starvation Driven Energy Expenditure Negatively Affects Ovarian Follicle Development" International Journal of Molecular Sciences 22, no. 7: 3557. https://doi.org/10.3390/ijms22073557

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