1. Introduction
Ionizing radiation (IR) has been widely used clinically in the treatment of a broad range of cancers over the past few decades [
1]. Cancer treatment techniques are focused on minimising the viability and migration (metastasis) of cancer cells. In radiation therapy, the effects of IR on the cells surrounding the tumour are of concern. Even though there is increased accuracy of targeting the cancerous tissues, those cells surrounding the tumour or in the path of the IR beam can become exposed to therapeutic radiation [
2,
3]. Therefore, it is vital to understand the mechanisms underlying the effects of IR on the viability and motility of both cancer and non-cancerous cells to improve these treatment techniques.
While IR has proven to be an effective tool in decreasing the viability of cells in a tumour mass [
4], its effects on cell migration remain controversial so that both promoting and inhibiting effects have been attributed to it [
5].
Different methods have been examined to improve radiation therapy by exploiting their radiation-induced cancer cell-killing while minimising the migration of these cells. One of these recent methods is the enclosure of metallic nanoparticles such as gold nanoparticles (AuNPs) in the cancer cell. The findings of such studies have shown that AuNPs have inhibitory effects on cancer cell migration without impacting on the viability of these cells which makes it a promising candidate for further application in gold nanoparticle aided radiotherapy (GNRT) [
6,
7,
8,
9]. Additionally, metallic nanoparticles have been used to enhance other cancer treatments methods such as magnetic hyperthermia and phototherapy [
10,
11,
12].
Despite extensive studies on the effects of IR and/or AuNPs on the viability and motility of cancer cells, very little is published about the percentage viability and relative migration of the corresponding non-cancerous cells when a solid tumour mass is exposed to a therapeutic IR dose in the presence or absence of AuNPs.
Based on the metabolic differences between non-cancerous and cancer cells [
13], in this in vitro study, we hypothesized that the same therapeutic dose of X-rays and the same concentration of AuNPs in the cells should result in different cell migration rates between tumour cells and their non-cancerous counterparts.
In order to be closer to the real radiation therapy conditions in both colon and melanoma cancers in which the normal tissues surrounding the tumour or in the path of the IR beam are exposed to the same dose of radiation, all the experiments were conducted using human SW48 colorectal adenocarcinoma and MM418-C1 melanoma cell lines and the results were compared to their corresponding non-cancerous counterparts, i.e., human CCD841 colon epithelial cells and epidermal melanocytes (HEM). In addition, due to dose enhancement and migration inhibiting effects of AuNPs combined with IR on cancer cells and based on the findings in our previous work [
14], the effects of 15 nm AuNPs combined with 5 Gy therapeutic X-ray on the viability and motility of these cancer cells and their non-cancerous counterparts were investigated.
3. Discussion and Conclusions
The majority of cancers (~90%) originate in epithelial tissues [
16] and most of the cancer-related deaths are caused by metastasis [
17]; therefore, understanding the role played by cell migration in epithelial cells is essential to enhance the effectiveness of IR in treating such tumours. The epithelium functions as a protective layer which is actively involved in the wound healing process [
18]. It is shown that normal cell behavior during the wound healing (migration) is similar to cancer metastasis and can be considered as two sides of the same coin [
18,
19,
20]. The similarity between wound healing and cancer metastasis is “vital” for radiation therapy because those non-cancerous cells surrounding the treatment area (along with cancer cells) are exposed to the same therapeutic radiation dose. In this in vitro study, it was anticipated that normal epithelial cell lines, i.e., CCD841 and HEM and their respective cancerous counterparts, i.e., SW48 and MM418-C1 cells after exposure to a clinical dose (5 Gy of X-ray) should respond differently with respect to cell migration and adherence. In our previous study we observed that pretreating the cells with 1 mM AuNPs 24 h prior to exposure to IR, while having minimal effects on cell viability did inhibit cell migration [
14], in this study, we examined the combined effects of AuNPs and IR on the viability and motility of two related cell lines that originate from the colon (CCD841 and SW48 cells) and the epidermis (HEM and MM418-C1 cells), to observe if these effects are tumour specific.
All cell lines incorporated gold intracellularly following exposure to AuNPs over a 24 h period (
Figure 1). While the levels taken up by the different cell lines were similar at concentrations < 1 mM, however, at higher concentrations (>1 mM) the tumour cell lines (SW48 and MM418-C1) did not incorporate any further gold in their cells, unlike that seen in their corresponding primary cell lines (CCD841 and HEM). Ivošev et al. [
21] also observed an increased uptake of gold from AuNPs in a range of tumour cell lines when compared to dermal fibroblasts, however they did not compare matched cell types (cancerous vs non-cancerous cells from the same cell type) as we did in this study. They observed that these tumours cells had different internalization pathways which may have explained the observed differences. Further studies using other matched cell lines from different regions of the body are warranted to observe if tumour cells take up more or less NPs that their corresponding non-cancerous cohorts. The results of which could have an impact on patients who are given AuNPs prior to exposure to therapeutic IR doses.
When the cells were treated with AuNPs, we observed no decrease in cell viability following incubated with 0–4 mM AuNPs over 48 h (
Figure 2). It was seen from
Figure 1, that at concentrations greater > 1 mM, the level of gold within the non-cancerous cells was proportional to the external concentration, however in the tumour cells intracellular levels did not increase above this concentration. These results are in agreement with that reported by Trono et al. [
22]. They observed that uptake and incorporation of AuNPs depend on various factors such as cell type (non-cancerous or cancerous), the concentration and size of the nanoparticles and the incubation period with the nanoparticles. In order to ensure that the internal concentration of gold was not having a direct effect on our studies all cell lines were treated with 1 mM AuNPs. When cells were pretreated with 1 mM AuNPs for 24 h and then subsequently exposed to 0–5 Gy X-rays (
Figure 4) there was no decrease in viability after 48 h when compared to those cells who were only exposed to IR (
Figure 3). A similar result was also observed when human prostate DU145 and lung A549 cancer cells were exposed to IR in the presence of absence of 1 mM AuNPs [
14].
Exposure to increasing doses of IR (0–6 Gy) resulted in a reduction in the viability of all four cell types over 48 h (
Figure 3). Similar effects were observed when DU145 prostate and A549 lung cancer cells were exposed to IR [
14]. The cytotoxic effects of IR were more pronounced on the tumour cells (SW48 and MM418-C1 cells) when compared to their non-cancerous counterparts (CCD841 and HEM). The difference in cell viability between these pairs of cells was ~15% following exposure to high doses of X-rays (e.g., the viability of HEM and MM418-C1 cells following exposure to 6 Gy was 78% and 60%, respectively). A similar result was also seen in CCD841 and SW48 exposed to the same IR dose resulting in 79% and 65% cell viability, respectively.
Different response to the IR and/or AuNPs was observed in cancer cells compared to that of their normal counterparts with regards to their migration rates. The migration of the tumour cell lines (SW48 and MM418-C1) were retarded when these cells were treated with either IR and/or AuNPs, while that of their non-cancerous counterparts (CCD841 and HEM) were enhanced when given the same treatments (
Figure 5,
Figure 6 and
Figure 7). This finding is in line with Moncharmont et al. [
5] who reported that IR can enhance or diminish cell migration in a range of tumour cell lines. The effects of AuNPs and that of IR on retarding the migration of both tumour cells (SW48 and MM418-C1) were similar (15–20%), however an additive effect (~35%) was seen when these cells were exposed to both treatments. This finding was similar to that seen when DU145 prostate and A549 lung cancer cells were treated with AuNPs and or IR [
14]. Of interest was that when the corresponding non-cancerous cells (CCD841 and HEM) were exposed to the same treatments cell migration was enhanced. In these cells, both AuNPs and IR individually enhanced cell migration by 13–16% but when they were exposed to both treatment migration increased by ~22%.
While both AuNPs and IR treatments impaired the migration of the tumour cells compared to their non-cancerous counterparts, AuNPs had no effect on adhesiveness of these cell lines (
Figure 8 and
Figure 9). IR enhanced the adhesiveness of the tumour cells (SW48 and MM418-C1 both ~230%) compared to their non-cancerous counterparts and this effect was proportional to the X-ray dose. This was similar to that seen when H1299 human non-small cell lung cancer cells [
23] and DU145 human prostate and A549 lung cancer cells [
14] were exposed to IR. When these tumour cells were pretreated with AuNPs no additive effect was observed in either the SW48 and MM418-C1 cells, which was similar to that seen in DU145 human prostate and A549 lung cancer cells [
14]. These changes to cell adhesion appear to be primarily mediated by IR as AuNPs had no effect on this process. A summary of the effects of AuNPs and IR on the migration and adhesiveness of the cells used in this study is shown in
Table 1.
The differences observed between the tumour cells and their non-cancerous counterparts do not appear to be related to acquired mutations. SW48 cells are colorectal adenocarcinoma cells and like the CCD841 colon epithelial cells they do not carry mutations for KRAS, NRAS, BRAF and PI3KCA [
24]. MM418-C1 cells possess the BRAF
V600E mutation and are homozygous for
CDKN2A deletions [
25], while HEM primary melanocytes do not carry these mutations.
Focal adhesions, podosomes and invadosomes located on the cell membrane play a major role in cell migration and adherence [
26]. These structures integrate external signals which cause cells to alter their morphology, so they can migrate and adhere to the substrate. Tsutsumi et al. [
23] observed that H1299 irradiated cells were more adhesive than unirradiated cells, which was due to increased numbers of focal adhesions on the cell membrane. They also observed an increase in matrix metalloprotease activity in the irradiated cells which would enhance cell migration. Following exposure to IR increased levels of phosphorylated focal adhesion kinases (FAK) as well as p38 and JNK was observed in lung A549 cancer cells [
27]. The addition of FAK inhibitors reduced the migration of radiation-induced medulloblastoma cells confirming the role these kinases play in IR-induced migration in these tumour cells, but not in non-cancerous cells. Further studies on the role played by FAK, p38 and JNK are warranted to see if there is a different response elicited between cancerous and non-cancerous cells when exposed to IR.
AuNPs on the other hand were shown to have a similar effect to that of IR on cell migration. Of interest that while it reduced tumour cell migration it enhanced that of the corresponding non-cancerous cells. Previous studies have shown the inhibitory effects of AuNPs on the migration of HEY A8 ovarian cancer cells [
6], DU145 human prostate and A549 lung cancer cells [
14]. As AuNPs are taken up into the cell, it has been shown that in HEY A8 cells they were trapped in the nuclear membrane which increased its stiffness which in turn reduced cell migration [
6], however it is unknown whether these particles have the same effect on non-cancerous cells. Recently, in examining mouse macrophages and mesenchymal stem cells, AuNPs interfered with the podosomes of these cells resulting reduced focal adhesions and ECM degradation in the macrophages while the opposite effect was seen in the stem cells [
28]. This cell specific effect of AuNPs on cell migration needs to be further investigated as we believe this is the first study to compare cancerous and non-cancerous cells of the same lineage, and highlights that differences exist between them. While we observed no effects of AuNPs on cell adhesion, Lo et al. [
29] found that naked AuNPs could reduce the adhesiveness of vascular smooth muscle cells by inhibiting FAK phosphorylation and actin cytoskeletal reorganisation. The authors used a range of AuNPs prepared by chemical or physical methods and this effect may be related to the nanoparticle itself. Further studies are warranted to investigate the effects AuNPs have on focal adhesions and podosomes as well as cell signalling pathways to determine how they interact with these cells. Similarly undertaking proteomic studies to observe what changes have occurred in the cell as a result of AuNPs and/or IR treatment is warranted, and along with the studies on changes in the activities of membrane signalling pathway intermediates will form the basis of suture studies. The main aim of this study was to investigate the combined effects of IR and AuNPs on two types of epithelial cells (colon—CCD841 and skin—HEM) and their cancerous counterparts (SW48 and MM418-C1).
Our results decisively showed that the therapeutic dose of IR separately and in combination with AuNPs significantly reduced the viability and migration rate of the tumour cells while having minor negative effects on the proliferation of their corresponding non-cancerous cohort while at the same time enhancing their migration rate. These findings are consistent with the effectiveness of radiation therapy in overall tumour control and highlight the benefits of utilizing AuNPs as a nontoxic and injectable agent in radiation therapy to improve its therapeutic outcomes. One of the major challenges facing radiotherapy is cancer metastasis, which are caused by cell migration. Hence, understanding testing, monitoring, and controlling metastasis could potentially result in more efficient radiotherapy. This research presents an efficient way of reducing cancer cell’s migration which could be utilised for controlling metastasis. Therefore, beside what has been well established about the role of NPs in enhancing radiation effects “dose” this work shows another role for such particles in radiotherapy and that is in reducing cancer cells migration which could lead to less possibilities for cancer metastasis. This could lead to vital changes in the regimen of radiation dose delivery in radiotherapy.
4. Materials and Methods
4.1. Cell Culture
Human colorectal adenocarcinoma cells (SW48: ATCC® CCL-231TM) and primary human colon epithelial cells (CCD841: ATCC® CRL-1790TM) were purchased from ATCC (Manassas, VA, USA). Human primary melanoma (MM418-C1) cells were a gift from Dr Glen Boyle (QIMR, Brisbane, Australia) while primary human epithelial melanocytes (HEM: ATCC® PCS-200-013TM) were purchased from ATCC and were used in this study.
SW48 cells were cultured and maintained in Leibovitz’s L-15 (Gibco®, Grand Island, NY, USA), 10% foetal bovine serum (FBS) (Gibco®) and 1% Penicillin-Streptomycin (Gibco®). CCD841 cells were cultured and maintained in EMEM (Gibco®), 10% FBS and 1% Penicillin-Streptomycin. MM418-C1 and HEM cells were cultured and maintained in RPMI 1640 (Gibco®), 10% FBS and 1% Penicillin-Streptomycin.
The CCD841, MM418-C1 and HEM cells were incubated at 37 °C with 5% CO2 in a humidified environment, while SW48 cells were maintained at 37 °C in a humidified environment and in a gas free exchange with atmosphere as CO2 and air mixture is detrimental to this cell line.
4.2. AuNPs Preparation
Spherical AuNPs were purchased from Nanoprobes (Yaphank, NY, USA). These AuNPs possess a metal core diameter of ~15 nm, which has been stabilized with a highly water-soluble thiol-based ligand [
30]. The AuNPs were stable in the culture media used in these experiments and no aggregation of the nanoparticles was observed after 48 h when media samples were observed under light microscopy using a 40× objective lens. The AuNPs solution was diluted using the appropriate cell culture medium to a final concentration of 0.197 mg/mL.
4.3. Inductively Coupled Plasma Mass Spectrometry (ICP MS) Measurement of Celluar AuNPs
Intracellular Au levels were determined using a ICP MS (Agilent 7700, Santa Clara, CA, USA). The cells (SW48, CCD841, MM418-C1 and HEM) were seeded (106 cells/well), in 6-well plates and incubated at 37 °C with 5% CO2 in a humidified environment for 24 h. After which, the cells were exposed to 0 to 4.0 mM AuNPs for 24 h. At the end of this period the cells were gently washed with warm PBS (37 °C), and then trypsinised. An aliquot of the resuspended cells was counted using a haemocytometer under a light microscope. The rest of the resuspended cells were dissolved in 1% HCl. In the ICP MS a calibration curve from a known gold standard (TraceCERT®, Sigma-Aldrich, St Louis, MO, USA) was established, and from which, the cellular uptake of the AuNPs was calculated and expressed as pg Au/cell.
4.4. Viability Assay
Cells (3 × 10
3 cells/well) were seeded in a 96-well plate incubated at 37 °C with 5% CO
2 in a humidified environment. After 24 h, the cells were treated with various concentrations of AuNPs ranging from 0 to 4.0 mM and/or exposed to 0 to 6 Gy of 6 MV X-rays.Cell viability was determined 48 h after treatment with AuNPs and/or IR, using the MTS assay as described previously [
14].
4.5. Cell Irradiation
The cells were irradiated with 6 MV X-ray generated by Linac (Elektra Synergy, Stockholm, Sweden) located at Australian Radiation Protection and Nuclear Safety Agency (ARPANSA), Yallambie, Australia. The radiation was delivered as a single fraction for each dose, i.e., 0 Gy for control groups and 1 to 6 Gy for treated groups, as described previously [
14]. The delivered dose was calculated from measurements using an ion chamber before the cell irradiation.
4.6. Scratch Assay
Cells were cultured in 6-well plates for 24 h until they reached ~90% confluency. The cells were washed with warm (37 °C) PBS before a sterile (200 μL) yellow pipette tip was used to create a ~600 µm gap. After this the wells were washes with warm PBS, and and fresh tissue culture media added. Gap filling was measured using the CytoSmart
® Live Image System (Piet Heinstraat, Zutphen, Holland) [
12]. The gap area at 0 h and 24 h were measured with ImageJ
® software and the relative migration was calculated using Equation (1) as seen below.
4.7. Adhesion Assay
Cellular adhesion (attachment) to the polystyrene surface of the well of a 6-well plate was evaluated in both control and treated groups. Cells grown in in 25 cm2 flasks were treated with either 1 mM AuNPs or exposed to 2 and/or 5 Gy 6 MV X-ray and incubated for 24 h. Where the cells were exposed to both treatments they were initially exposed to 1 mM AuNPs for 24 h before being irradiated with either 2 and/or 5 Gy 6 MV X-ray and incubated for a further 24 h. At the end of this period, each experimental group, i.e., control (untreated), irradiated with 2 or 5 Gy and/or treated with AuNPs were trypsinised and 2 × 104 cells seeded in 6-well plates. After 4 h incubation at 37 °C, the non-adherent cells were gently washed with warm PBS (37 °C) and images were taken using an EVOS® XL Cell Imaging System (Thermo Fisher Scientific, Waltham, WA, USA). The adherent cells in four different random 6.25 × 104 µm2 areas (250 × 250 µm square region) were determined and the average values plotted.
4.8. Statistical Analysis
All presented data within this paper are the mean of at least three independent experiments. Statistical comparison between the control group and AuNPs group, IR group, and AuNPs + IR group were performed using one-way analysis of variance (ANOVA) with IBM SPSS Statistics version 25 (IBM Australia Ltd., St Leonards, NSW, Australia). Results are reported as mean ± standard error of the mean (SEM). * p < 0.05 were considered statistically significant.