Next Article in Journal
Prominent Indomethacin-Induced Enteropathy in Fcgriib Defi-cient lupus Mice: An Impact of Macrophage Responses and Immune Deposition in Gut
Previous Article in Journal
Extracellular Vesicles Do Not Mediate the Anti-Inflammatory Actions of Mouse-Derived Adipose Tissue Mesenchymal Stem Cells Secretome
Previous Article in Special Issue
Microbial Virulence Factors
Open AccessArticle

Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence in Candida glabrata

1
Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal
2
Biological Sciences Research Group, IBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, 1049-001 Lisbon, Portugal
3
Medical Mycology Research Center (MMRC), Chiba University, Chiba 263-8522, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Academic Editor: Makoto Kimura
Int. J. Mol. Sci. 2021, 22(3), 1376; https://doi.org/10.3390/ijms22031376
Received: 21 December 2020 / Revised: 18 January 2021 / Accepted: 23 January 2021 / Published: 29 January 2021
(This article belongs to the Special Issue Microbial Virulence Factors)
Candida glabrata is an emerging fungal pathogen whose success depends on its ability to resist antifungal drugs but also to thrive against host defenses. In this study, the predicted multidrug transporter CgTpo4 (encoded by ORF CAGL0L10912g) is described as a new determinant of virulence in C. glabrata, using the infection model Galleria mellonella. The CgTPO4 gene was found to be required for the C. glabrata ability to kill G. mellonella. The transporter encoded by this gene is also necessary for antimicrobial peptide (AMP) resistance, specifically against histatin-5. Interestingly, G. mellonella’s AMP expression was found to be strongly activated in response to C. glabrata infection, suggesting AMPs are a key antifungal defense. CgTpo4 was also found to be a plasma membrane exporter of polyamines, especially spermidine, suggesting that CgTpo4 is able to export polyamines and AMPs, thus conferring resistance to both stress agents. Altogether, this study presents the polyamine exporter CgTpo4 as a determinant of C. glabrata virulence, which acts by protecting the yeast cells from the overexpression of AMPs, deployed as a host defense mechanism. View Full-Text
Keywords: Candida glabrata; CgTpo4; virulence; Galleria mellonella; AMP resistance; polyamine resistance Candida glabrata; CgTpo4; virulence; Galleria mellonella; AMP resistance; polyamine resistance
Show Figures

Figure 1

MDPI and ACS Style

Cavalheiro, M.; Romão, D.; Santos, R.; Mil-Homens, D.; Pais, P.; Costa, C.; Galocha, M.; Pereira, D.; Takahashi-Nakaguchi, A.; Chibana, H.; Fialho, A.M.; Teixeira, M.C. Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence in Candida glabrata. Int. J. Mol. Sci. 2021, 22, 1376. https://doi.org/10.3390/ijms22031376

AMA Style

Cavalheiro M, Romão D, Santos R, Mil-Homens D, Pais P, Costa C, Galocha M, Pereira D, Takahashi-Nakaguchi A, Chibana H, Fialho AM, Teixeira MC. Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence in Candida glabrata. International Journal of Molecular Sciences. 2021; 22(3):1376. https://doi.org/10.3390/ijms22031376

Chicago/Turabian Style

Cavalheiro, Mafalda; Romão, Daniela; Santos, Rui; Mil-Homens, Dalila; Pais, Pedro; Costa, Catarina; Galocha, Mónica; Pereira, Diana; Takahashi-Nakaguchi, Azusa; Chibana, Hiroji; Fialho, Arsénio M.; Teixeira, Miguel C. 2021. "Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence in Candida glabrata" Int. J. Mol. Sci. 22, no. 3: 1376. https://doi.org/10.3390/ijms22031376

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop