Diversified Biomineralization Roles of Pteria penguin Pearl Shell Lectins as Matrix Proteins
Abstract
:1. Introduction
2. Results
2.1. Knockdown Analysis of PPL3 and PPL4 in the Biomeneralization Processes
2.2. Molecular Properties of PPL3 and PPL4 on In Vitro Crystallization
2.3. Effect of Carbohydrates on the Biomeneralization Processes of PPL3 and PPL4
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Isolation of Lectins from Secretory Fluid of Mantle
4.3. Morpholino Design and Morphological Phenotype Analysis of Morphants of P. penguin D-Shaped Larva
4.4. In Vitro Crystallization
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
A | Alexa Fluor |
BSA | bovine serum albumin |
CRD | carbohydrate recognition domain |
Fuc | fucose |
GlcNAc | N-acetyl-D-glucosamine |
HPLC | high performance liquid chromatography |
JRL | jacalin related lectin |
Man | mannose |
MO | morpholino oligo |
MS | mass spectrometry |
SDS-PAGE | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
SEM | scanning electron microscopy |
Xyl | xylose |
M3FX | Manαl~6(Manαl~3)(Xylβl~2)Manβl~4GlcNAcβ1~4(Fucαl~3)GlcNAc- |
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Ogawa, T.; Sato, R.; Naganuma, T.; Liu, K.; Sato, S.; Sakaue, S.; Osada, M.; Yoshimi, K.; Muramoto, K. Diversified Biomineralization Roles of Pteria penguin Pearl Shell Lectins as Matrix Proteins. Int. J. Mol. Sci. 2021, 22, 1081. https://doi.org/10.3390/ijms22031081
Ogawa T, Sato R, Naganuma T, Liu K, Sato S, Sakaue S, Osada M, Yoshimi K, Muramoto K. Diversified Biomineralization Roles of Pteria penguin Pearl Shell Lectins as Matrix Proteins. International Journal of Molecular Sciences. 2021; 22(3):1081. https://doi.org/10.3390/ijms22031081
Chicago/Turabian StyleOgawa, Tomohisa, Rie Sato, Takako Naganuma, Kayeu Liu, Saho Sato, Shizuka Sakaue, Makoto Osada, Kyosuke Yoshimi, and Koji Muramoto. 2021. "Diversified Biomineralization Roles of Pteria penguin Pearl Shell Lectins as Matrix Proteins" International Journal of Molecular Sciences 22, no. 3: 1081. https://doi.org/10.3390/ijms22031081