Calcite Nanotuned Chitinous Skeletons of Giant Ianthella basta Marine Demosponge
Abstract
:1. Introduction
2. Results
2.1. Identification of Ca and Br Localization Using Three-Dimensional Confocal Micro X-ray Fluorescence (CµXRF)
2.2. Visualization of the Presence of Mineral Phase Using Electron Microscopy Methods
2.3. Identification of Calcite within I. basta Skeletal Fibres
2.4. Near Edge X-ray Fine Structure (NEXAFS) Features of Identified Mineral Phase
3. Discussion and Conclusions
4. Materials and Methods
4.1. Supply of Demosponge Specimens
4.2. Preparation of Demsoponge Samples for Analysis
4.3. Digital, Light, and Fluorescence Microscopy
4.4. FTIR
4.5. Raman
4.6. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDS)
4.7. Transmission Electron Microscopy (TEM)
4.8. Near Edge X-ray Fine Structure (NEXAFS)
4.9. XRD Analysis
4.10. Confocal Micro X-ray Flourescence (CµXRF)
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Kertmen, A.; Petrenko, I.; Schimpf, C.; Rafaja, D.; Petrova, O.; Sivkov, V.; Nekipelov, S.; Fursov, A.; Stelling, A.L.; Heimler, K.; et al. Calcite Nanotuned Chitinous Skeletons of Giant Ianthella basta Marine Demosponge. Int. J. Mol. Sci. 2021, 22, 12588. https://doi.org/10.3390/ijms222212588
Kertmen A, Petrenko I, Schimpf C, Rafaja D, Petrova O, Sivkov V, Nekipelov S, Fursov A, Stelling AL, Heimler K, et al. Calcite Nanotuned Chitinous Skeletons of Giant Ianthella basta Marine Demosponge. International Journal of Molecular Sciences. 2021; 22(22):12588. https://doi.org/10.3390/ijms222212588
Chicago/Turabian StyleKertmen, Ahmet, Iaroslav Petrenko, Christian Schimpf, David Rafaja, Olga Petrova, Viktor Sivkov, Sergey Nekipelov, Andriy Fursov, Allison L. Stelling, Korbinian Heimler, and et al. 2021. "Calcite Nanotuned Chitinous Skeletons of Giant Ianthella basta Marine Demosponge" International Journal of Molecular Sciences 22, no. 22: 12588. https://doi.org/10.3390/ijms222212588