Next Article in Journal
Salivary Trefoil Factor Family (TFF) Peptides and Their Roles in Oral and Esophageal Protection: Therapeutic Potential
Previous Article in Journal
Complex Carbohydrates and Glycoconjugates: Structure, Functions and Applications
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 22
10.3390/ijms222212220
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.)

by
Liqun Tang
1,†,
Huayu Xu
1,†,
Yifeng Wang
1,
Huimei Wang
1,
Zhiyong Li
1,
Xixi Liu
1,
Yazhou Shu
1,
Guan Li
1,
Wanning Liu
1,
Jiezheng Ying
1,
Xiaohong Tong
1,
Jialing Yao
2,
Wenfei Xiao
3,
Shaoqing Tang
1,*,
Shen Ni
1 and
Jian Zhang
1,*
1
State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
2
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
3
Institute of Biotechnology, Hangzhou Academy of Agriculture Sciences, Hangzhou 310024, China
*
Authors to whom correspondence should be addressed.
†
These authors contribution equally to this work.
Int. J. Mol. Sci. 2021, 22(22), 12220; https://doi.org/10.3390/ijms222212220
Submission received: 14 July 2021 / Revised: 4 August 2021 / Accepted: 12 August 2021 / Published: 12 November 2021
(This article belongs to the Section Molecular Plant Sciences)
Browse Figures
Review Reports Versions Notes

Abstract

:
Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive. Here, we report the functional characterization of a GA-inducible OsABF1 in GA biosynthesis underpinning plant height and seed germination. Overexpression of OsABF1 produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination. Meanwhile, the phenotypes could be rescued by exogenous GA3, suggesting that OsABF1 is a key regulator of GA homeostasis. OsABF1 could directly suppress the transcription of green revolution gene SD1, thus reducing the endogenous GA level in rice. Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to OsABF1 overexpression lines. It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the SD1 promoter, thus epigenetically silencing SD1 to maintain the GA homeostasis for growth and seed germination. These findings shed new insight into the functions of OsABF1 and regulatory mechanism underlying GA homeostasis in rice.

1. Introduction

Increasing crop production has been an eternal theme of the world. Previous findings implied that gibberellins (GAs) are major regulators for crop growth and yield through optimizing the harvest index and lodging resistance [1]. In addition, the dormancy and germination process of plant seeds are closely related to GA content, which is a key development stage in crop life cycle and an important factor determining grain yield and quality [2]. As a class of diterpenoids hormones, GAs participate in nearly all important plant growth and developmental events, such as stem and hypocotyl elongation, leaf expansion, seed dormancy and flowering [3,4]. According to the number of carbon atoms in the GA molecule, GAs may be divided into two types: Carbon-20 GAs and Carbon-19 GAs [5]. Thus far, GA biosynthesis pathways have been well documented and have become fairly straightforward. Firstly, in the ent-kaurene (the first committed intermediate in GA biosynthesis) biosynthesis progress, geranylgeranyl pyrophosphate (GGPP) is converted into ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) [6]. Secondly, after several oxidation reactions, ent-kaurene produces GA12-aldehyde [7]. Finally, GA12-aldehyde is converted into different GA intermediates and bioactive GAs by GA20-oxidase (GA20ox) and GA3-oxidase (GA3ox), respectively.
Defects of the GA biosynthesis pathways genes usually lead to typical GA-deficient phenotypes such as dwarf, weak germination vigor, and late flowering [8,9,10,11]. One of the most famous examples is SD1 (semi-dwarf 1) encoding an oxidase enzyme in GA biosynthesis [12]. Mutations in the SD1 reduced the plant height, which conferred rice with lodging resistance and higher harvest index, and finally led to the “green revolution” in rice breeding [12]. It has been known that GA20ox converts C-20 from a methyl group to an aldehyde by sequential oxidations and then removes a carbon atom to produce C19-GAs (GA9 and GA20) [13]. GA20ox genes have been identified in numerous model plant species, including Arabidopsis [14,15,16] and rice [17,18]. Two types of GA20ox have been found: OsGA20ox1 and OsGA20ox2 [12,19]. Except for the “Green Revolution Gene”, another GA20ox gene, OsGA20ox1, also has been identified with similar functions to that of the OsGA20ox2. Overexpression of OsGA20ox1 leads to a taller plant statue, which is a typical GA-overproduction phenotype [20]. Other than the GA biosynthesis genes, large numbers of transcription factors participate in the GAs biosynthesis. For example, GA20ox1, which determines the optimal elongation of petiole and hypocotyl under high temperature, can be used as the target gene of transcription factor TCP15 in Arabidopsis thaliana [21], while MADS-box transcription factor SHORT VEGETATIVE PHASE (SVP) inhibits flowering through directly repressing transcription of AtGA20ox2 [22]. Similarly, Tobacco RSG (REPRESSION OF SHOOT GROWTH) encoding a bZIP (Basic leucine zipper) transcription activator also controls GA biosynthetic enzyme NtGA20ox1, underpinning cell elongation and shoot growth [23,24].
bZIP transcription factors, as highly conserved types of protein, are widely distributed in eukaryotes. There are about 110 and 89 bZIP transcription factors in Arabidopsis and rice, respectively [25,26]. The differences in structural feature divide bZIPs into 10 subfamilies with diverse functions [26,27,28]. For example, group A is mainly involved in abscisic acid (ABA) or stress signaling [29,30]. As transcription factors, bZIPs can directly bind to the conserved G-box cis elements (CACGTG) in the promoter to activate or repress the transcription of target genes, which underpins various biological processes, including GA biosynthesis and signaling [30,31,32,33,34]. For example, OsbZIP48 is a homolog of Arabidopsis AtHY5 controlling photomorphogenesis. Overexpression of OsbZIP48 in rice reduced the plant height seriously. Further research revealed that OsbZIP48 regulates GA homeostasis by directly binding to the promoter of OsKO2 that encodes an ent-kaurene oxidase 2 of the gibberellin biosynthesis pathway [35]. Several other bZIPs such as ABI3, ABI4, and ABI5 may also directly or indirectly affect GA accumulation or signaling through ABA signaling pathways [35,36,37,38,39,40,41]. Previous research revealed that OsABF1 (LOC_Os01g64730), an ABA responsive element binding factor, represses rice flowering through indirectly suppressing the transcription of Early heading date 1 (Ehd1) [42]. OsABF1 was also found to be involved in numbers of abiotic and biotic stress responses, including salt stress, drought stress, and ABA responding, which are very important to plant growth and development [42,43,44]. In this study, we explored the functional characterization of OsABF1 in GA biosynthesis underpinning plant height and seed germination. OsABF1 could directly suppress the transcription of green revolution gene SD1, thus determining the endogenous GA level in rice. Overexpression of OsABF1 in rice produced semi-dwarf phenotype with retarded seed germination, which could be restored by exogenous GA. Moreover, OsABF1 interacts with OsEMF2b to recruit PRC2-mediated H3K27me3 deposition on the SD1 promoter, thus epigenetically silencing SD1 to maintain the GA homeostasis for growth and seed germination. The rice germplasms with overexpression of OsABF1, which caused semi-dwarf phenotype and with retarded seed germination, could be used in rice breeding with lodging resistance and pre-harvest sprouting resistance.

2. Results

2.1. OsABF1 Overexpression Lines Exhibited GA-Deficient Phenotypes

Two representative overexpression lines, OxABF1-6 and OxABF1-9, with over 300 folds upregulation were used for phenotypical characterization (Figure S1a). Compared with wild-type (WT), the OxABF1-6 and OxABF1-9 lines showed obvious dwarfism, which was a typical GA-deficient phenotype (Figure 1a). By comparing the length of the internodes of OxABF1-6, OxABF1-9, and WT, we found that all the internodes of OsABF1 overexpression lines were shortened by 20% to 30%, which finally made the height of OsABF1 overexpression lines only 73% of that of the WT (Figure 1b,c). To figure out the reason for the semi-dwarf phenotype of OxABF1-6 plants, we employed paraffin sections to observe the cell size in the second internodes of the OxABF1-6 and the wild-type (Figure 1d,g). The cell length and width of OxABF1-6 was significantly decreased as compared with the WT (Figure 1i,j). Moreover, the number of large vascular bundles (LVB) and small vascular bundles (SVB) were also reduced in OxABF1-6 (Figure 1e,f), indicating that the dwarfism of OxABF1-6 could be attributed to the reduced cell size and proliferation (Figure 1h). Additionally, we performed seed germination assays on OsABF1 overexpression seeds and WT. OsABF1 overexpression lines showed obviously lower germination rate and post-germination growth than the WT (Figure 2a). More importantly, exogenous GA3 fully rescued the OxABF1-6 and OxABF1-9 germination rates to the WT level, and also partially recovered its post-germination growth (Figure 2c,d,f,g). Meanwhile, application of paclobutrazol (PAC, an endogenous GA biosynthesis inhibitor) significantly inhibited the seed germination rate and post-germination growth of WT seeds to the OxABF1-6 level but showed no significant effects on those of the OxABF1-6 (Figure 2b,f,g). Furthermore, hormone quantification assay confirmed that the GA3 in 15 days seedlings of OxABF1-6 reduced over 50% compared with the WT (Figure 2e). Meanwhile, the other endogenous GAs were also quantified, and the results showed that the level of GA5, GA8, GA13, GA20, GA24, and GA34 were significantly decreased in OxABF1-6 (Figure S1b). Thus, we concluded that the observed retarded seed germination and seedling growth of OxABF1 lines were due to GA deficiency.
Employing the CRISPR/Cas9 technique, we successfully knocked out OsABF1 and simultaneously knocked out OsABF1 and its closest homolog bZIP40 (LOC_Os05g36160). The obtained mutants harbored different types of insertions or deletions in the coding sequence of the targeted genes, some of which shifted the open reading frames and reduced the transcriptional levels (Figure S1c,d). However, both abf1 and abf1 bzip40 mutants showed no substantial difference with the WT in plant height and seed germination, suggesting that OsABF1 might be functionally redundant with bZIP40 and other bZIP sibling genes (Figure 1c,d and Figure S2).

2.2. OsABF1 Directly Repressed the Transcription of SD1

The GA-deficient phenotype of OsABF1 overexpression lines suggested that OsABF1 participated in the regulation of GA biosynthesis or catabolism pathways. Indeed, qRT-PCR analysis revealed that the transcription level of OsABF1 increased threefold at 1 HAT (hours after GA treatment) and then decreased rapidly at 3 HAT in young seedlings (Figure 3a). Subsequently, the expression levels of several GA-related genes were checked in OxABF1-6 and the WT by qRT-PCR. As a result, KS1 (LOC_Os04g52230), KOS1 (LOC_Os06g37300), KOS2 (LOC_Os06g37224), GA2ox3 (LOC_Os01g55240), and GA2ox5 (LOC_Os07g01340) were upregulated in OxABF1-6, whereas SD1 (GA20ox2, LOC_Os01g66100) and GA2ox1 (LOC_Os05g06670) were downregulated in OxABF1-6 (Figure 3b).
As a crucial gene in GA biosynthesis and the green revolution in rice breeding, SD1 attracted our particular attention due to its downregulation pattern and the existence of a conserved bZIP binding G-box cis element in its promoter [45] (Figure 3c). In order to check whether SD1 is a direct target of OsABF1, we performed yeast one-hybrid (Y1H) experiment. In the yeast, OsABF1 successfully activated the expression of proSD1::LacZ reporter, thus OsABF1 may directly bind to the SD1 promoter (Figure 3e). Moreover, we conducted electrophoresis mobility shift assay (EMSA) to test the binding in vitro. The GST-OsABF1 protein could physically bind to the SD1a probe containing the G-box, and the excess unlabeled competitive probes effectively alleviated the biotin probe bound by GST-ABF1 (Figure 3d). ChIP-qPCR result further demonstrated that OsABF1 was significantly enriched in the regions P3 of SD1 promoter where the G-box cis element was located (Figure 3f). Finally, we performed luciferase (LUC) transient transcriptional activity assay to detect the effect of OsABF1 on SD1 transcription in rice protoplasts. The result showed that the luciferase activity of the proSD1::LUC reporter was significantly repressed when OsABF1 was introduced. The result indicated that OsABF1 acted as a transcription repressor on SD1, which consistent with the reduced SD1 expression in OsABF1 overexpression lines (Figure 3g,h). Taken together, we proposed that OsABF1 could directly bind to the SD1 promoter and repress its transcription to regulate GA biosynthesis.

2.3. OsABF1 Bound to OsEMF2b, Causing the Deposition of H3K27me3 on the SD1 Promoter

Polycomb group (PcG) proteins have been shown to regulate growth and development in plants via mediating H3K27me3-suppressing histone modification on the target genes, which has been recognized as a significant epigenetic mechanism in gene regulation [46,47]. The PcG proteins in rice include two E(z)-like homologues (OsCLF and OsiEZ1), two Su(z)12 homologues (OsEMF2a and OsEMF2b), and two ESC homologues (OsFIE1 and OsFIE2), among which OsEMF2b has been reported to control plant height and seed germination by regulating the expression of OsVP1 [48,49]. As mentioned above, the overexpression of OsABF1 reduced the plant height and seed germination; therefore, we speculated that OsABF1 may interact with OsEMF2b to recruit PcG-mediated repression on SD1. The hypothesis was tested by yeast two-hybrid assays, which showed that OsABF1-BD physically binds to OsEMF2b-AD in yeast (Figure 4a). In the GST pull-down assay, the purified His-OsEMF2b was pulled down with GST-OsABF1, but not with GST tag, indicating that OsABF1 interacts with OsEMF2b in vitro (Figure 4c). The BiFC experiment in plant also revealed that the nYFP-OsEMF2b and OsABF1-cYFP pair produced strong fluorescent signals in the nucleus of tobacco leaf cells, whereas no fluorescence signals were observed when nYFP-OsEMF2b or OsABF1-cYFP was expressed alone (Figure 4b). Therefore, these results strongly demonstrated that OsABF1 binds to OsEMF2b. Finally, we performed ChIP-qPCR to test the deposition patterns of OsEMF2b and its mediated histone modification H3K27me3 on the SD1 region. The results showed that both OsEMF2b and H3K27me3 were significantly enriched in the SD1 region, particularly the P3 region, which is largely overlapped with the OsABF1 deposition patterns (Figure 4d,e). Hence, OsABF1 represses the SD1 transcription via recruiting the PcG-mediated H3K27me3.

2.4. OsEMF2b and OsABF1 Had Antagonistic Roles in GA Biosynthesis Regulation

In contrast to the GA-induced expression pattern of OsABF1, the transcription of OsEMF2b was significantly downregulated by exogenous GA in young seedlings at 1 HAT, suggesting the antagonistic roles of OsABF1 and OsEMF2b in GA biosynthesis regulation (Figure 3a). We adopted an OsEMF2b T-DNA insertional mutant from another lab for the characterization of its biological function [47]. The emf2b contains a T-DNA insertion in the fourth exon, which largely disrupted the expression of the gene (Figure S1e). The homozygous emf2b mutants displayed a similar but more severe dwarf phenotype than OsABF1 overexpression lines (Figure 5a–c). Unfortunately, we were not able to test the seed germination of emf2b mutants due to the complete sterility of the homozygous emf2b lines (Figure S1f). In emf2b, OsABF1 was significantly elevated (Figure 5d). Meanwhile, OsEMF2b expression was drastically reduced in OsABF1 overexpression lines (Figure 5e). We also performed qRT-PCR to examine the expression of GA-related genes in emf2b and the WT and found that all the tested genes showed the same differential expression pattern in emf2b as in OsABF1 overexpression lines (Figure 5f). In particular, GA20ox2/SD1 expression in emf2b was decreased to only 1% of that in the WT, which well explained the extremely dwarf nature of emf2b.

3. Discussion

3.1. OsABF1 Targets SD1 to Regulate GA Biosynthesis

The growth and development of plants are regulated by many factors in vivo and vitro, including light, temperature, water, and genetic factors. The plant hormones are of the most importance in regulating the growth and development, for which the biosynthetic pathways are adjusted by many transcription factors. In this study, we revealed that a bZIP transcription factor OsABF1 regulates GA biosynthesis via targeting the green revolution gene SD1. Although the knock-out mutants of OsABF1 and the double mutants abf1bzip40 grew as normal as the WT (Figure S2), the overexpression of OsABF1 gave rise to typical GA-deficient phenotypes such as semi-dwarf and later seed germination (Figure 1a–c and Figure 2a). Meanwhile, overexpression of OsABF1 exhibited significantly lower GA3 concentration than the WT (Figure 2e). More importantly, the application of exogenous GA3 successfully restored the phenotype of OsABF1 overexpression lines to the WT level, suggesting that GA deficiency is the major reason for the observed phenotypes (Figure 2c,d). Actually, OsABF1 has also been reported as a suppressor of rice flowering [42]. Given that GA is a major hormone promoting plant flowering, the later flowering phenotype should be attributed to the GA deficiency in OsABF1 overexpression lines as well. Moreover, Y1H, EMSA, and LUC transient transactivation and ChIP-qPCR assays confirmed that OsABF1 could directly bind to the G-box cis elements in the promoter regions of SD1 to repress its transcription, which strongly suggested that SD1 is a key node of the OsABF1-governed GA homeostasis (Figure 3c–h).
SD1 has been identified as an oxidase enzyme in GA biosynthesis. Mutations in the SD1 led to the “green revolution” in rice breeding by reducing the plant height with stronger lodging resistance and higher harvest index [12]. Meanwhile, the qSD1-2, another allele of SD1, was found involved in the reduction of GA synthesis, seed dormancy enhancement, and plant height reduction [8]. In breeding practice, double-stranded RNAi on two SD1 fragments influenced the plant height in different extents [50]. Gene editing on SD1 enabled the rapid improvement of landraces with shorter plant height and enhanced lodging resistance without sacrificing many other excellent agronomic traits such as low phosphorus tolerance and broad-spectrum resistance to a variety of diseases and insect pests [51].

3.2. OsABF1 May Mediate the Antagonistic Relationship of ABA and GA

Like indispensable lubrication in plants lifecycle, various plant hormones regulate plant growth and development synergistically or antagonistically, their relationship woven into a series of complex networks in plants [32,52,53,54]. Abscisic acid (ABA) and GA work antagonistically in regulating various plant growth and development events such as seed dormancy and germination, root growth, plant height, flowering initiation, and abiotic stress [11,55,56]. It was found that exogenous ABA usually reduces GA accumulation, while ABA-deficient or ABA-signaling mutants were able to bypass the GA requirement for seed germination [57]. Thus far, a large number of mediators of ABA–GA antagonism have been identified. In Arabidopsis, ABF4 significantly increased the level of ABA and promoted the transcriptional regulation of GA metabolism genes, which suggested that ABF4 was a hub of ABA and GA signaling pathway [58]. It was revealed that SnRK2s-APC/CTE regulatory module mediated the antagonism between GA and ABA signal transduction in higher plants [59]. A very recent study from our lab also reported that rice WRKY72 inhibits GA accumulation and seed germination via the “WRKY72-LRK1-OsKO2” pathway [60]. Given that WRKY72 is a phosphorylation substrate protein of the ABA signaling core component SAPK10 [61], WRKY72 may serve as a key node in the ABA–GA interaction. Actually, several pieces of evidence have shown that OsABF1 is functionally implicated in ABA signaling. Transcription of OsABF1 is significantly induced by ABA and is able to bind to the ABA-responsive cis elements [44]. Its mutants became more sensitive to drought and salinity treatments, which are closely related to the functions of ABA. Additionally, its closest Arabidopsis orthologs, OsABF1, 2, 3, and 4, are also ABA-inducible and functionally involved in abiotic stress responses [62]. Combining the roles of OsABF1 in ABA signaling and GA biosynthesis suppression, it is rational to propose that OsABF1 is a key player in mediating the antagonistic relationship of ABA and GA.

3.3. OsABF1 Interacts with OsEMF2b-PRC2 Complex to Suppress SD1 Transcription through H3K27me3

Higher eukaryotes have evolved profound epigenetic regulatory mechanisms to fine-tune gene transcription. Initially identified in Drosophila, PcG proteins have been reported to inactivate gene expression and maintain the silencing state of the target chromatin through covalent histone modifications [63,64]. PcG proteins contain polycomb repressive complex 1 (PRC1) and PRC2. PRC1 complexes are E3 ubiquitin ligases depositing mono ubiquitylation of lysine 119 mark on histone 2A (H2AK119ub), while PRC2 majorly catalyzes tri-methylation on histone H3 at lysine 27 (H3K27me3) to repress target transcription [48,49,64]. PRC2 is not able to bind to the target inherent DNA, due to lack of DNA binding domains. Thus, its localization to target sites largely relies on other transcription factors that can link PRC2 and the DNA motifs [65]. Recently, recruitment of PRC2 by transcription factors has been demonstrated as a significant mechanism in gene transcriptional regulation. For example, in 2016, two independent labs reported that VAL1 and VAL2 bound to the RY motifs in the FLC promoter, wherein they recruit PRC2-mediated H3K27me3 for FLC repression and flowering promotion after vernalization [66,67]. Similarly, OsLF recruits PRC2 to the CANNTG (bHLH cis-element) motif of Hd1 by interacting with PRC2 component OsVIL2 and represses the Hd1 expression via H3K27 tri-methylation modification [68]. In the current study, we demonstrated that OsABF1 recruits EMF2b-mediated H3K27me3 on the promoter of SD1, thus epigenetically regulating GA biosynthesis. This conclusion has been supported by several lines of evidence including (1) OsABF1 being a direct repressor of SD1 (Figure 3c–e); (2) OsABF1 being able to physically interact with PRC2 component OsEMF2b, in vitro and vivo (Figure 4a–c); and (3) the enriched regions of OsABF1, OsEMF2b, and H3K27me3 in the SD1 promoter largely overlapping (Figure 3f and Figure 4d,e). Together with other reports mentioned above, our study suggests that plant growth and development may subject to a very general regulatory mechanism that transcription factors repress target gene transcription via recruiting PRC2-mediated H3K27me3 modification.
In addition, there might be a regulation mechanism that overexpression of OsABF1 could repress the expression of SD1. In the meantime, enrichment of OsEMF2b would mediate more H3K27me3 in the promoter of SD1, and also repress its expression. Further, in plants, there must be a regulation mechanism of maintaining the GA homeostasis, namely, the moderate and stable expression of SD1. Thus, when OsABF1 was overexpressed, then the expression of OsEMF2b was reduced (Figure 5e), whereas when OsEMF2b mutant to emf2b, then the expression of OsABF1 was significantly increased to repress the expression of SD1 (Figure 5d) and then maintain the moderate and stable expression and the GA homeostasis in plant.

4. Materials and Methods

4.1. Plant Materials and Growth Conditions

The complete coding sequence of OsABF1 was amplified using the cDNA of Nipponbare (Oryza sativa ssp. japonica) leaf and inserted into the fusion vector pCAMBIA1390 driven by the ubiquitin promoter with restriction digestion site of KpnI and BamHI. To produce OsABF1 mutant lines, we used a reported CRISPR/Cas9 technique to generate abf1 and abf1bzip40 double mutants [69]. All the binary vectors were transformed into the WT (japonica cv. Nipponbare) by Agrobacterium tumefaciens-mediated method.
The emf2b (A T-DNA insertion mutant of OsEMF2b) lines in japonica cv. Dongjin were adopted from other labs [50]. To measure plant height and test seed germination rate, we cultivated all plants during the normal rice-growing season in the experimental field of China National Rice Research Institute, Hangzhou (119°57′ E, 30°05′ N). The primers and sgRNA sequences are provided in Table S1.

4.2. Seed Germination and Seedling Growth Assays

The experiment of seed germination was conducted on the basis of the previous study with minor revision [32]. Briefly, all of the dehulled seeds were sterilized in 70% ethanol for 2 min, then in 50% NaClO for 40 min, and washed in sterilized water until clean. Then, the seeds were placed on 1/2 strength Murashige and Skoog medium (pH 5.8) and incubated in a growth cabinet (28 ± 2 °C, 12/12 h photoperiod and 70% relative humidity) for 7 days. As the seed coleoptile reached 5 mm in length, this phenotype was defined as complete germination. Seed germination rate was recorded every 12 h, and three biological replications (each replicate including 50 seeds) were used for each sample. Seedling height was measured after 7 days of germination. For the hormone treatment, different concentrations of GA3 and PAC were added into 1/2 strength Murashige and Skoog medium treatment.

4.3. Hormone Quantification

For GAs quantification in Nipponbare and OxABF1-6 plants, 3 g of 15-days old fresh seedlings of Nipponbare and OxABF1-6 were harvested and then frozen in liquid nitrogen. Quantification of endogenous GAs levels was measured by liquid chromatography–tandem mass spectrometry system. This method was conducted as described in previous studies, with a few modifications [70,71]. All samples were extracted with 1.5 mL methanol with concentration of 80% at 4 °C for 16 h, then centrifuged at 13,000× g at 4 °C for 10 min. Further, the supernatant was collected and evaporated to dryness under a nitrogen gas stream. Finally, it was reconstituted in 100 mL of 95% acetonitrile for the liquid chromatography–tandem mass spectrometry analysis.

4.4. Paraffin Sections

Internodes of WT and OxABF1-6 collected at post-heading stage were soaked in FAA (70% ethanol 90 mL, 5 mL acetic acid, and 37–40% formaldehyde (5 mL)) solution for about 24 h, then dehydrated through gradient ethanol concentrations followed by different graded chloroform concentrations. Finally, all the treated samples were soaked in wax, embedded and sliced according to the previous study [72]. All tissues were stained with Safranine O-Fast Green Stain Kit (YESEN Biotech, Shanghai, China) and sliced into 10-mm sections using a microtome (Leica, Wetzlar, Germany). Images were photographed using a Leica DM2500 microscope (Leica, Wetzlar, Germany). The cell size was measured by ImageJ software (ImageJ V.1.8.0).

4.5. Quantitative Reverse Transcription (qRT)-PCR

RNA isolation kit (YESEN Biotech, Shanghai, China) was used to extract total RNA. cDNAs were synthesized by Prime Script RT Master Mix (Takara, Dalian, China) and quantitative real-time PCR of various genes was conducted by CFX 96 real-time PCR instrument (Bio-Rad, California, USA). Each sample was performed with three technical repetitions, and UBQ (LOC_Os03g13170) was used as internal control. The transcription levels were calculated by the 2−△△CT method. Primers used in this study were listed in Table S1.

4.6. Yeast One-Hybrid Assay

Yeast one-hybrid system (Clontech, CA, USA) was used to check the interaction between OsABF1 and the SD1 promoter. The complete coding sequence of OsABF1 was fused to the pB42AD vector with EcoRI and XhoI sites. The promoter of SD1 (1500 bp upstream of the transcriptional start site) was amplified and inserted into the pLacZi vector using EcoRI and KpnI sites. All fused vectors were introduced into EGY48 (yeast strain), then grown on SD/-Ura/-Trp plates.

4.7. Electrophoresis Mobility Shift Assay (EMSA)

The probes of sd1 were synthesized and labeled with Cy5.5 by SUNYA biotech (Hangzhou, China). Non-labeled probes were used as competitors. For the Cy5.5-labeled probe, all the process of the EMSA was performed under darkness. Odyssey CLx infrared fluorescence imaging system (LI-COR, Lincoln, NE, USA) was used to detect the fluorescence signal of the EMSA gel. Probes used were listed in Table S1.

4.8. Luciferase Transient Transcriptional Activity Assay

For the luciferase activity assay, 1500bp upstream of the SD1 transcription start site was amplified and inserted into 190LUC vector with HindIII and BglII to generate the reporter. The CDS of OsABF1 was fused into “None” with BamHI and EcoRI as an effector. Both fusion vector plasmids were co-transformed into rice protoplast, and the luciferase activity was analyzed by the Luciferase Reporter Assay System (Promega, Madison, WI, USA). Rice protoplast extraction and transformation were conducted according to a predecessor study [73]. Primers used were listed in Table S1.

4.9. ChIP-qPCR

The ChIP-qPCR assay was conducted following the previously published protocol [48]. OsABF1 and OsEMF2b antibodies were commercially synthesized by Gene script company (Shanghai, China), and H3K27me3 antibody was purchased (Cat No: Ab6002. Abcam, Cambridge, MA, USA). Chromatin was extracted from 3 g of 15 DAG (days after germination) OsABF1 overexpression seedlings and then cross-linked with 1% formaldehyde under vacuum. Then, the extracted chromatin was fragmented to about 300 bps in size by sonication. Finally, DNA/protein complex was immuno-precipitated with polyclonal OsABF1, OsEMF2b, and H3K27me3 antibodies. Triple biological replicates of each sample were performed, and the data analysis was conducted according to previously reported methods [46,49]. Primers used were listed in Table S1.

4.10. Yeast Two-Hybrid Analysis and Bimolecular Fluorescence Complementation

The yeast two-hybrid assay was conducted on the basis of the manufacturer’s protocol (Clontech, Palo Alto, CA, USA). The full-length CDS of OsABF1 and OsEMF2b were amplified and separately fused into bait vector pGBKT7 and prey vector pGADT7. pGBKT7-OsABF1 and pGADT7-OsEMF2b were co-transformed into yeast strain Y2H Gold. pGBKT7-53 and pGADT7-T were used as positive controls, pGBKT7 and pGADT7 were used as negative controls. For the bimolecular fluorescence complementation (BiFC) experiment, OsABF1 CDS without stop codon was fused into pDOE vector at the BspeI site, and the complete coding sequence of OsEMF2b was inserted into pDOE vector at the BamHI site. Then, the fusional expression vectors were transformed into Agrobacterium strain EHA105 by electroporation.

4.11. In Vitro Pull-Down Assay

For the vitro pull-down assay, the full CDS of the OsABF1 and OsEMF2b were amplified and ligated into the pGEX-4T-1 and pET28a vectors, respectively. The fused constructs and empty pGEX-4T-1 were introduced into E. coli strain Transetta (Transgen, Beijing, China). The primers were provided in Table S1.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ijms222212220/s1.

Author Contributions

Conceptualization, J.Z.; formal analysis, G.L.; investigation, H.X.; methodology, Z.L., X.L., Y.S. and X.T.; project administration, S.T.; resources, J.Y. (Jialing Yao) and J.Z.; software, Y.W., H.W., W.L., W.X. and S.N.; supervision, J.Y. (Jiezheng Ying); writing—original draft, L.T.; writing—review and editing, J.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by National Natural Science Foundation of China (grant no. 32071986), National Key R&D Program of China (2020YFE0202300), CNRRI key research and development project (CNRRI-2020-01), and ASTIP program of CAAS.

Conflicts of Interest

The authors declared no conflict of interest.

References

  1. Gao, S.; Chu, C. Gibberellin Metabolism and Signaling: Targets for Improving Agronomic Performance of Crops. Plant Cell Physiol. 2020, 61, 1902–1911. [Google Scholar] [CrossRef]
  2. Shu, K.; Liu, X.D.; Xie, Q.; He, Z.H. Two Faces of One Seed: Hormonal Regulation of Dormancy and Germination. Mol. Plant 2016, 9, 34–45. [Google Scholar] [CrossRef] [Green Version]
  3. Achard, P.; Genschik, P. Releasing the brakes of plant growth: How GAs shutdown DELLA proteins. J. Exp. Bot. 2009, 60, 1085–1092. [Google Scholar] [CrossRef] [Green Version]
  4. Olszewski, N.; Sun, T.P.; Gubler, F. Gibberellin signaling: Biosynthesis, catabolism, and response pathways. Plant Cell 2002, 14, S61–S80. [Google Scholar] [CrossRef] [Green Version]
  5. Silverstone, A.L.; Sun, T. Gibberellins and the Green Revolution. Trends Plant Sci. 2000, 5, 1–2. [Google Scholar] [CrossRef]
  6. Sponsel, V.M. The localization, metabolism and biological activity of gibberellins in maturing and germinating seeds of Pisum sativum cv. Progress No. 9. Planta 1983, 159, 454–468. [Google Scholar] [CrossRef]
  7. Graebe, J.E. Gibberellin Biosynthesis from Gibberellin A12-Aldehyde; Springer: Berlin/Heidelberg, Germany, 1986. [Google Scholar]
  8. Ye, H.; Feng, J.; Zhang, L.; Zhang, J.; Mispan, M.S.; Cao, Z.; Beighley, D.H.; Yang, J.; Gu, X.Y. Map-Based Cloning of Seed Dormancy1–2 Identified a Gibberellin Synthesis Gene Regulating the Development of Endosperm-Imposed Dormancy in Rice. Plant Physiol. 2015, 169, 2152–2165. [Google Scholar]
  9. Annunziata, M.G. The Long and the Short of It: GA 2-oxidaseA9 Regulates Plant Height in Wheat. Plant Physiol. 2018, 177, 3–4. [Google Scholar] [CrossRef] [Green Version]
  10. Ge, W.; Steber, C.M. Positive and negative regulation of seed germination by the Arabidopsis GA hormone receptors, GID1a, b, and c. Plant Direct. 2018, 2, e00083. [Google Scholar] [CrossRef]
  11. Chen, H.; Ruan, J.; Chu, P.; Fu, W.; Liang, Z.; Li, Y.; Tong, J.; Xiao, L.; Liu, J.; Li, C.; et al. AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds. Plant J. 2020, 101, 310–323. [Google Scholar] [CrossRef]
  12. Sasaki, A.; Ashikari, M.; Ueguchi-Tanaka, M.; Itoh, H.; Nishimura, A.; Swapan, D.; Ishiyama, K.; Saito, T.; Kobayashi, M.; Khush, G.S.; et al. Green revolution: A mutant gibberellin-synthesis gene in rice. Nature 2002, 416, 701–702. [Google Scholar] [CrossRef]
  13. Hedden, P.; Phillips, A.L. Gibberellin metabolism: New insights revealed by the genes. Trends Plant Sci. 2000, 5, 523–530. [Google Scholar] [CrossRef]
  14. Withanage, S.P.; Hossain, M.A.; Kumar, M.S.; Roslan, H.A.; Abdullah, M.P.; Napis, S.B.; Shukor, N.A. Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants. Breed. Sci. 2015, 65, 177–191. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Plackett, A.R.; Powers, S.J.; Fernandez-Garcia, N.; Urbanova, T.; Takebayashi, Y.; Seo, M.; Jikumaru, Y.; Benlloch, R.; Nilsson, O.; Ruiz-Rivero, O.; et al. Analysis of the developmental roles of the Arabidopsis gibberellin 20-oxidases demonstrates that GA20ox1, -2, and -3 are the dominant paralogs. Plant Cell 2012, 24, 941–960. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Rieu, I.; Ruiz-Rivero, O.; Fernandez-Garcia, N.; Griffiths, J.; Powers, S.J.; Gong, F.; Linhartova, T.; Eriksson, S.; Nilsson, O.; Thomas, S.G.; et al. The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle. Plant J. 2008, 53, 488–504. [Google Scholar] [CrossRef] [PubMed]
  17. Jia, X.; Yu, L.; Tang, M.; Tian, D.; Yang, S.; Zhang, X.; Traw, M.B. Pleiotropic changes revealed by in situ recovery of the semi-dwarf gene sd1 in rice. Plant Physiol. 2020, 248, 153141. [Google Scholar] [CrossRef] [PubMed]
  18. Qin, X.; Liu, J.H.; Zhao, W.S.; Chen, X.J.; Guo, Z.J.; Peng, Y.L. Gibberellin 20-oxidase gene OsGA20ox3 regulates plant stature and disease development in rice. Plant Microbe Interact. 2013, 26, 227–239. [Google Scholar] [CrossRef] [Green Version]
  19. Toyomasu, T.; Kawaide, H.; Sekimoto, H.; Von Numers, C.; Phillips, A.L.; Hedden, P.; Kamiya, Y. Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings. Physiol. Plantarum. 2010, 99, 111–118. [Google Scholar] [CrossRef]
  20. Oikawa, T.; Koshioka, M.; Kojima, K.; Yoshida, H.; Kawata, M. A role of OsGA20ox1, encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice. Plant Mol. Biol. 2004, 55, 687–700. [Google Scholar] [CrossRef]
  21. Ferrero, V.; Viola, I.L.; Ariel, F.D.; Gonzalez, D.H. Class I TCP Transcription Factors Target the Gibberellin Biosynthesis Gene GA20ox1 and the Growth-Promoting Genes HBI1 and PRE6 during Thermomorphogenic Growth in Arabidopsis. Plant Cell Physiol. 2019, 60, 1633–1645. [Google Scholar] [CrossRef]
  22. Andrés, F.; Porri, A.; Torti, S.; Mateos, J.; Romera-Branchat, M.; García-Martínez, J.L.; Fornara, F.; Gregis, V.; Kater, M.M.; Coupland, G. SHORT VEGETATIVE PHASE reduces gibberellin biosynthesis at the Arabidopsis shoot apex to regulate the floral transition. Proc. Natl. Acad. Sci. USA 2014, 111, E2760–E2769. [Google Scholar] [CrossRef] [Green Version]
  23. Fukazawa, J.; Nakata, M.; Ito, T.; Yamaguchi, S.; Takahashi, Y. The transcription factor RSG regulates negative feedback of NtGA20ox1 encoding GA 20-oxidase. Plant J. 2010, 62, 1035–1045. [Google Scholar]
  24. Fukazawa, J.; Sakai, T.; Ishida, S.; Yamaguchi, I.; Kamiya, Y.; Takahashi, Y. Repression of shoot growth, a bZIP transcriptional activator, regulates cell elongation by controlling the level of gibberellins. Plant Cell 2000, 12, 901–915. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. E, Z.G.; Zhang, Y.P.; Zhou, J.H.; Wang, L. Mini review roles of the bZIP gene family in rice. Genet. Mol. Res. 2014, 13, 3025. [Google Scholar] [CrossRef] [PubMed]
  26. Dröge-Laser, W.; Snoek, B.L.; Snel, B.; Weiste, C. The Arabidopsis bZIP transcription factor family-an update. Curr. Opin. Plant Biol. 2018, 45, 36–49. [Google Scholar] [CrossRef]
  27. Jakoby, M.; Weisshaar, B.; Dröge-Laser, W.; Vicente-Carbajosa, J.; Tiedemann, J.; Kroj, T.; Parcy, F. bZIP transcription factors in Arabidopsis. Trends Plant Sci. 2002, 7, 106–111. [Google Scholar] [CrossRef]
  28. Nijhawan, A.; Jain, M.; Tyagi, A.K.; Khurana, J.P. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol. 2008, 146, 333–350. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Liu, J.; Chu, J.; Ma, C.; Jiang, Y.; Ma, Y.; Xiong, J.; Cheng, Z.M. Overexpression of an ABA-dependent grapevine bZIP transcription factor, VvABF2, enhances osmotic stress in Arabidopsis. Plant Cell Rep. 2019, 38, 587–596. [Google Scholar] [CrossRef]
  30. Yang, S.; Xu, K.; Chen, S.; Li, T.; Xia, H.; Chen, L.; Liu, H.; Luo, L. A stress-responsive bZIP transcription factor OsbZIP62 improves drought and oxidative tolerance in rice. BMC Plant Biol. 2019, 19, 260. [Google Scholar] [CrossRef] [Green Version]
  31. Zinsmeister, J.; Lalanne, D.; Terrasson, E.; Chatelain, E.; Vandecasteele, C.; Vu, B.L.; Dubois-Laurent, C.; Geoffriau, E.; Signor, C.L.; Dalmais, M.; et al. ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes. Plant Cell 2016, 28, 2735–2754. [Google Scholar] [CrossRef] [Green Version]
  32. Wang, Y.; Hou, Y.; Qiu, J.; Wang, H.; Wang, S.; Tang, L.; Tong, X.; Zhang, J. Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10-bZIP72-AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa). New Phytol. 2020, 228, 1336–1353. [Google Scholar] [CrossRef]
  33. Giuliano, G.; Pichersky, E.; Malik, V.S.; Timko, M.P.; Scolnik, P.A.; Cashmore, A.R. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc. Natl. Acad. Sci. USA 1988, 85, 7089–7093. [Google Scholar] [CrossRef] [Green Version]
  34. Donald, R.G.; Schindler, U.; Batschauer, A.; Cashmore, A.R. The plant G box promoter sequence activates transcription in Saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to GBF, the plant G box binding factor. EMBO J. 1990, 9, 1727–1735. [Google Scholar] [CrossRef]
  35. Shu, K.; Zhang, H.; Wang, S.; Chen, M.; Wu, Y.; Tang, S.; Liu, C.; Feng, Y.; Cao, X.; Xie, Q. ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis. PLoS Genet. 2013, 9, e1003577. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Burman, N.; Bhatnagar, A.; Khurana, J.P. OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development. Plant Physiol. 2018, 176, 1262–1285. [Google Scholar] [CrossRef] [PubMed]
  37. Skubacz, A.; Daszkowska-Golec, A.; Szarejko, I. The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk. Front. Plant Sci. 2016, 7, 1884. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Hussain, S.; Kim, S.H.; Bahk, S.; Ali, A.; Nguyen, X.C.; Yun, D.J.; Chung, W.S. The Auxin Signaling Repressor IAA8 Promotes Seed Germination Through Down-Regulation of ABI3 Transcription in Arabidopsis. Front. Plant Sci. 2020, 11, 111. [Google Scholar] [CrossRef] [Green Version]
  39. Lin, J.H.; Yu, L.H.; Xiang, C.B. ARABIDOPSIS NITRATE REGULATED 1 acts as a negative modulator of seed germination by activating ABI3 expression. New Phytol. 2020, 225, 835–847. [Google Scholar] [CrossRef]
  40. Utsugi, S.; Ashikawa, I.; Nakamura, S.; Shibasaka, M. TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination. J. Plant Res. 2020, 133, 245–256. [Google Scholar] [CrossRef]
  41. Hu, Y.; Han, X.; Yang, M.; Zhang, M.; Pan, J.; Yu, D. The Transcription Factor INDUCER OF CBF EXPRESSION1 Interacts with ABSCISIC ACID INSENSITIVE5 and DELLA Proteins to Fine-Tune Abscisic Acid Signaling during Seed Germination in Arabidopsis. Plant Cell 2019, 31, 1520–1538. [Google Scholar] [CrossRef]
  42. Zhang, C.; Liu, J.; Zhao, T.; Gomez, A.; Li, C.; Yu, C.; Li, H.; Lin, J.; Yang, Y.; Liu, B.; et al. A Drought-Inducible Transcription Factor Delays Reproductive Timing in Rice. Plant Physiol. 2016, 171, 334–343. [Google Scholar] [CrossRef] [Green Version]
  43. Zhang, C.; Li, C.; Liu, J.; Lv, Y.; Yu, C.; Li, H.; Zhao, T.; Liu, B. The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice. J. Exp. Bot. 2017, 68, 4695–4707. [Google Scholar] [CrossRef] [Green Version]
  44. Amir Hossain, M.; Lee, Y.; Cho, J.I.; Ahn, C.H.; Lee, S.K.; Jeon, J.S.; Kang, H.; Lee, C.H.; An, G.; Park, P.B. The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice. Plant Mol. Biol. 2010, 72, 557–566. [Google Scholar] [CrossRef] [PubMed]
  45. Spielmeyer, W.; Ellis, M.H.; Chandler, P.M. Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc. Natl. Acad. Sci. USA 2002, 99, 9043–9048. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Liu, X.; Zhou, C.; Zhao, Y.; Zhou, S.; Wang, W.; Zhou, D.X. The rice enhancer of zeste [E(z)] genes SDG711 and SDG718 are respectively involved in long day and short day signaling to mediate the accurate photoperiod control of flowering time. Front. Plant Sci. 2014, 5, 591. [Google Scholar] [CrossRef] [Green Version]
  47. Yang, J.; Lee, S.; Hang, R.; Kim, S.R.; Lee, Y.S.; Cao, X.; Amasino, R.; An, G. OsVIL2 functions with PRC2 to induce flowering by repressing OsLFL1 in rice. Plant J. 2013, 73, 566–578. [Google Scholar] [CrossRef] [PubMed]
  48. Chen, M.; Xie, S.; Ouyang, Y.; Yao, J. Rice PcG gene OsEMF2b controls seed dormancy and seedling growth by regulating the expression of OsVP1. Plant Sci. 2017, 260, 80–89. [Google Scholar] [CrossRef]
  49. Zhong, J.; Peng, Z.; Peng, Q.; Cai, Q.; Peng, W.; Chen, M.; Yao, J. Regulation of plant height in rice by the Polycomb group genes OsEMF2b, OsFIE2 and OsCLF. Plant Sci. 2018, 267, 157–167. [Google Scholar] [CrossRef]
  50. Qiao, F.; Zhao, K.-J. The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice. Plant Mol. Biol. Rep. 2011, 29, 952–960. [Google Scholar] [CrossRef]
  51. Hu, X.; Cui, Y.; Dong, G.; Feng, A.; Wang, D.; Zhao, C.; Zhang, Y.; Hu, J.; Zeng, D.; Guo, L.; et al. Using CRISPR-Cas9 to generate semi-dwarf rice lines in elite landraces. Sci. Rep. 2019, 9, 19096. [Google Scholar] [CrossRef] [Green Version]
  52. Liu, X.; Hu, P.; Huang, M.; Tang, Y.; Li, Y.; Li, L.; Hou, X. The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis. Nat. Commun. 2016, 7, 12768. [Google Scholar] [CrossRef] [Green Version]
  53. Barreto, L.C.; Herken, D.M.D.; Silva, B.M.R.; Munné-Bosch, S.; Garcia, Q.S. ABA and GA(4) dynamic modulates secondary dormancy and germination in Syngonanthus verticillatus seeds. Planta 2020, 251, 86. [Google Scholar] [CrossRef]
  54. Shuai, H.; Meng, Y.; Luo, X.; Chen, F.; Zhou, W.; Dai, Y.; Qi, Y.; Du, J.; Yang, F.; Liu, J.; et al. Exogenous auxin represses soybean seed germination through decreasing the gibberellin/abscisic acid (GA/ABA) ratio. Sci. Rep. 2017, 7, 12620. [Google Scholar] [CrossRef] [Green Version]
  55. Verma, V.; Ravindran, P.; Kumar, P.P. Plant hormone-mediated regulation of stress responses. BMC Plant Biol. 2016, 16, 86. [Google Scholar] [CrossRef] [Green Version]
  56. Vishal, B.; Kumar, P.P. Regulation of Seed Germination and Abiotic Stresses by Gibberellins and Abscisic Acid. Front. Plant Sci. 2018, 9, 838. [Google Scholar] [CrossRef] [PubMed]
  57. Shu, K.; Zhou, W.; Yang, W. APETALA 2-domain-containing transcription factors: Focusing on abscisic acid and gibberellins antagonism. New Phytol 2018, 217, 977–983. [Google Scholar] [CrossRef]
  58. Muñiz García, M.N.; Stritzler, M.; Capiati, D.A. Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation. Planta 2014, 239, 615–631. [Google Scholar] [CrossRef]
  59. Lin, Q.; Wu, F.; Sheng, P.; Zhang, Z.; Zhang, X.; Guo, X.; Wang, J.; Cheng, Z.; Wang, J.; Wang, H.; et al. The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways. Nat. Commun. 2015, 6, 7981. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  60. Wang, H.; Hou, Y.; Wang, S.; Tong, X.; Wang, Y. WRKY72 Negatively Regulates Seed Germination Through Interfering Gibberellin Pathway in Rice. Rice Sci. 2021, 28, 1–5. [Google Scholar]
  61. Hou, Y.; Wang, Y.; Tang, L.; Tong, X.; Wang, L.; Liu, L.; Huang, S.; Zhang, J. SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance. iScience 2019, 16, 499–510. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Omidvar, V.; Abdullah, S.; Chai, L.H.; Mahmood, M.; Al-Shanfari, A.B. Isolation and characterization of two ABRE-binding proteins: EABF and EABF1 from the oil palm. Mol. Biol. Rep. 2012, 39, 8907–8918. [Google Scholar] [CrossRef]
  63. Bondos, S.E.; Hsiao, H.C. Roles for Intrinsic Disorder and Fuzziness in Generating Context-specific Function in Ultrabithorax, a Hox Transcription Factor. Adcances Exp. Med. Biol. 2012, 725, 86–105. [Google Scholar]
  64. Yan, B.; Lv, Y.; Zhao, C.; Wang, X. Knowing When to Silence: Roles of Polycomb-Group Proteins in SAM Maintenance, Root Development, and Developmental Phase Transition. Int. J. Mol. Sci. 2020, 21, 5871. [Google Scholar] [CrossRef]
  65. Xiao, J.; Jin, R.; Yu, X.; Shen, M.; Wagner, J.D.; Pai, A.; Song, C.; Zhuang, M.; Klasfeld, S.; He, C.; et al. Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis. Nat. Genet. 2017, 49, 1546–1552. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Qüesta, J.I.; Song, J.; Geraldo, N.; An, H.; Dean, C. Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization. Science 2016, 353, 485–488. [Google Scholar] [CrossRef] [PubMed]
  67. Yuan, W.; Luo, X.; Li, Z.; Yang, W.; Wang, Y.; Liu, R.; Du, J.; He, Y. A cis cold memory element and a trans epigenome reader mediate Polycomb silencing of FLC by vernalization in Arabidopsis. Nat. Genet. 2016, 48, 1527–1534. [Google Scholar] [CrossRef] [PubMed]
  68. Wang, J.; Hu, J.; Qian, Q.; Xue, H.W. LC2 and OsVIL2 promote rice flowering by photoperoid-induced epigenetic silencing of OsLF. Mol. Plant 2013, 6, 514–527. [Google Scholar] [CrossRef] [Green Version]
  69. Ma, X.; Zhang, Q.; Zhu, Q.; Liu, W.; Chen, Y.; Qiu, R.; Wang, B.; Yang, Z.; Li, H.; Lin, Y.; et al. A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. Mol. Plant 2015, 8, 1274–1284. [Google Scholar] [CrossRef]
  70. Tang, Y.; Liu, H.; Guo, S.; Wang, B.; Li, Z.; Chong, K.; Xu, Y. OsmiR396d Affects Gibberellin and Brassinosteroid Signaling to Regulate Plant Architecture in Rice. Plant Physiol. 2018, 176, 946–959. [Google Scholar] [CrossRef] [Green Version]
  71. Chen, Y.; Dan, Z.; Gao, F.; Chen, P.; Fan, F.; Li, S. Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism. Plant Physiol. 2020, 184, 393–406. [Google Scholar] [CrossRef]
  72. Zhao, J.; Long, T.; Wang, Y.; Tong, X.; Tang, J.; Li, J.; Wang, H.; Tang, L.; Li, Z.; Shu, Y.; et al. RMS2 Encoding a GDSL Lipase Mediates Lipid Homeostasis in Anthers to Determine Rice Male Fertility. Plant Physiol. 2020, 182, 2047–2064. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. He, Y.; Shi, Y.; Zhang, X.; Xu, X.; Wu, J.L. The OsABCI7 transporter interacts with OsHCF222 to stabilize the thylakoid membrane in rice. Plant Physiol. 2020, 184, 283–299. [Google Scholar] [CrossRef] [PubMed]
Ijms 22 12220 g001 550
Figure 1. The plant height and length of internodes were reduced in OsABF1 overexpression plants. (a) Plant morphology of OxABF1-6, abf1bzip40-1, and WT (Nipponbare) at the start heading stage, scale bar = 20 cm. (b) The panicle and internodes of OxABF1-6, OxABF1-9, abf1bzip40, and WT (Nipponbare). Bar = 2 cm. (c) Statistical analysis of plant height and internode length of OxABF1-6 and WT (Nipponbare). Data are shown as means ± SD (n = 15). (d) Cross-section of the second internode in WT and OxABF1-6, scale bar = 200 μm. SVB: small vascular bundle; LVB: large vascular bundle. (e,f) Number of SVB and LVB calculated from cross-sections of the second internode; values are shown as means ± SD (n = 20). (g) Longitudinal section of the second internode in WT and OxABF1-6, scale bar = 200 μm. (h) Cell numbers of the second internodes of WT and OxABF1-6; values are shown as means ± SD (n = 20). (i,j) The parenchyma cell (PC) length and width. Data is shown as means ± SD (n = 100). Throughout this figure, ** p < 0.01 by Student’s t-test analysis.
Figure 1. The plant height and length of internodes were reduced in OsABF1 overexpression plants. (a) Plant morphology of OxABF1-6, abf1bzip40-1, and WT (Nipponbare) at the start heading stage, scale bar = 20 cm. (b) The panicle and internodes of OxABF1-6, OxABF1-9, abf1bzip40, and WT (Nipponbare). Bar = 2 cm. (c) Statistical analysis of plant height and internode length of OxABF1-6 and WT (Nipponbare). Data are shown as means ± SD (n = 15). (d) Cross-section of the second internode in WT and OxABF1-6, scale bar = 200 μm. SVB: small vascular bundle; LVB: large vascular bundle. (e,f) Number of SVB and LVB calculated from cross-sections of the second internode; values are shown as means ± SD (n = 20). (g) Longitudinal section of the second internode in WT and OxABF1-6, scale bar = 200 μm. (h) Cell numbers of the second internodes of WT and OxABF1-6; values are shown as means ± SD (n = 20). (i,j) The parenchyma cell (PC) length and width. Data is shown as means ± SD (n = 100). Throughout this figure, ** p < 0.01 by Student’s t-test analysis.
Ijms 22 12220 g001
Ijms 22 12220 g002 550
Figure 2. Seed germination characteristics of OsABF1 overexpression lines. (a–d) Seed germination time courses of the WT, OxABF1-6, and OxABF1-9 under mock (a), 10 µM PAC treatment (b), 1.5 µM GA3 (c), and 2 µM GA3 (d). (e) Quantification of GA derivatives of WT and OxABF1-6 seedlings analyzed with liquid chromatography–tandem mass spectrometry. Error bars represent means ± SD (n = 3), ** indicates statistically significant difference at p < 0.01 compared with Nipponbare in Student’s t-test. (f,g) After 7 days seed germination, phenotypes and seedlings height of the WT, OxABF1–6, and OxABF1–9 under mock, 10 µM PAC treatment, 1.5 µM GA3, and 2 µM GA3. Error bars represent means ± SD (n = 20). ** p < 0.01 by Student’s t-test analysis.
Figure 2. Seed germination characteristics of OsABF1 overexpression lines. (a–d) Seed germination time courses of the WT, OxABF1-6, and OxABF1-9 under mock (a), 10 µM PAC treatment (b), 1.5 µM GA3 (c), and 2 µM GA3 (d). (e) Quantification of GA derivatives of WT and OxABF1-6 seedlings analyzed with liquid chromatography–tandem mass spectrometry. Error bars represent means ± SD (n = 3), ** indicates statistically significant difference at p < 0.01 compared with Nipponbare in Student’s t-test. (f,g) After 7 days seed germination, phenotypes and seedlings height of the WT, OxABF1–6, and OxABF1–9 under mock, 10 µM PAC treatment, 1.5 µM GA3, and 2 µM GA3. Error bars represent means ± SD (n = 20). ** p < 0.01 by Student’s t-test analysis.
Ijms 22 12220 g002
Ijms 22 12220 g003 550
Figure 3. OsABF1 directly bound to the SD1 promoter and repressed its transcription. (a) Time-course expression level of OsABF1 in 15-day-old seedlings upon 100 μM exogenous GA3 treatment. HAT: hours after GA treatment. (b) The expression levels of GA-related genes in WT and OxABF1-6 leaves at tillering stage. (c) Schematic presentation of the SD1 promoter regions (1500bp upstream of the SD1 transcription start site). Bar 1 to 6 indicate the regions checked by ChIP-qPCR. The red asterisk (SD1a) represents the position of the G-box motif. (d) EMSA assay showed OsABF1 directly binds to the promoter of SD1. The 1×, 10×, 25×, 50×, and 100× fold excess non-labeled probes were used for competition. The red letter represented G-box. (e) Yeast one-hybrid assay showed that OsABF1 could bind to the SD1 promoter (1500 bps upstream of the SD1 transcription start site); UDT1-pB42AD and RMS2-pLacZi were used as CK+. (f) ChIP-qPCR analysis of the OsABF1 enrichment on SD1 promoter in OxABF1-6. (g,h) Luciferase (LUC) transient transcriptional activity assay in rice protoplast. Error bars represent means ± SD (n = 3). * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Figure 3. OsABF1 directly bound to the SD1 promoter and repressed its transcription. (a) Time-course expression level of OsABF1 in 15-day-old seedlings upon 100 μM exogenous GA3 treatment. HAT: hours after GA treatment. (b) The expression levels of GA-related genes in WT and OxABF1-6 leaves at tillering stage. (c) Schematic presentation of the SD1 promoter regions (1500bp upstream of the SD1 transcription start site). Bar 1 to 6 indicate the regions checked by ChIP-qPCR. The red asterisk (SD1a) represents the position of the G-box motif. (d) EMSA assay showed OsABF1 directly binds to the promoter of SD1. The 1×, 10×, 25×, 50×, and 100× fold excess non-labeled probes were used for competition. The red letter represented G-box. (e) Yeast one-hybrid assay showed that OsABF1 could bind to the SD1 promoter (1500 bps upstream of the SD1 transcription start site); UDT1-pB42AD and RMS2-pLacZi were used as CK+. (f) ChIP-qPCR analysis of the OsABF1 enrichment on SD1 promoter in OxABF1-6. (g,h) Luciferase (LUC) transient transcriptional activity assay in rice protoplast. Error bars represent means ± SD (n = 3). * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Ijms 22 12220 g003
Ijms 22 12220 g004 550
Figure 4. OsABF1 recruits PRC2 complex to target loci by interacting with OsEMF2b. (a) Yeast two-hybrid assay showed OsABF1 interaction with OsEMF2b in yeast. Yeast cells co-transformed with OsABF1-BD and OsEMF2b-AD were grown on SD-Trp/-Leu/-Ade/-His selective medium with 3 mM 3-AT (a competitive inhibitor of the HIS3 gene product). (b) BiFC was used to detect the interaction between OsABF1 and OsEMF2b. The tested protein pairs were constructed in vector pDOE-BiFC and transiently expressed in tobacco leaf epidermal cells. Positive interactions are indicated by the visualization of YFP fluorescence, scale bar = 20 µm. (c) In vitro pull-down assay. Purified His-OsEMF2b and GST-OsABF1 were subjected to pull-down assays and detected with anti-His and anti-GST antibodies, respectively. (d,e) ChIP-qPCR analysis of OsEMF2b and H3K27me3 deposition on SD1 target loci in OxABF1-6. Values are means ± SD (n = 3). * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Figure 4. OsABF1 recruits PRC2 complex to target loci by interacting with OsEMF2b. (a) Yeast two-hybrid assay showed OsABF1 interaction with OsEMF2b in yeast. Yeast cells co-transformed with OsABF1-BD and OsEMF2b-AD were grown on SD-Trp/-Leu/-Ade/-His selective medium with 3 mM 3-AT (a competitive inhibitor of the HIS3 gene product). (b) BiFC was used to detect the interaction between OsABF1 and OsEMF2b. The tested protein pairs were constructed in vector pDOE-BiFC and transiently expressed in tobacco leaf epidermal cells. Positive interactions are indicated by the visualization of YFP fluorescence, scale bar = 20 µm. (c) In vitro pull-down assay. Purified His-OsEMF2b and GST-OsABF1 were subjected to pull-down assays and detected with anti-His and anti-GST antibodies, respectively. (d,e) ChIP-qPCR analysis of OsEMF2b and H3K27me3 deposition on SD1 target loci in OxABF1-6. Values are means ± SD (n = 3). * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Ijms 22 12220 g004
Ijms 22 12220 g005 550
Figure 5. The plant height of emf2b was reduced. (a) The phenotype of WT (Dongjin) and emf2b. (b,d) OsEMF2b and OsABF1 transcriptional level in WT (Dongjin) and emf2b leaves at tillering stage. (c) The plant height of WT (Dongjin) and emf2b. (e) OsEMF2b transcriptional level in WT (Nipponbare) and OxABF1-6 leaves at tillering stage. (f) The expression levels of GA-related genes in WT (Dongjin) and emf2b leaves at tillering stage. Throughout this figure, * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Figure 5. The plant height of emf2b was reduced. (a) The phenotype of WT (Dongjin) and emf2b. (b,d) OsEMF2b and OsABF1 transcriptional level in WT (Dongjin) and emf2b leaves at tillering stage. (c) The plant height of WT (Dongjin) and emf2b. (e) OsEMF2b transcriptional level in WT (Nipponbare) and OxABF1-6 leaves at tillering stage. (f) The expression levels of GA-related genes in WT (Dongjin) and emf2b leaves at tillering stage. Throughout this figure, * p < 0.05, ** p < 0.01 by Student’s t-test analysis.
Ijms 22 12220 g005
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Tang, L.; Xu, H.; Wang, Y.; Wang, H.; Li, Z.; Liu, X.; Shu, Y.; Li, G.; Liu, W.; Ying, J.; et al. OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2021, 22, 12220. https://doi.org/10.3390/ijms222212220

AMA Style

Tang L, Xu H, Wang Y, Wang H, Li Z, Liu X, Shu Y, Li G, Liu W, Ying J, et al. OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.). International Journal of Molecular Sciences. 2021; 22(22):12220. https://doi.org/10.3390/ijms222212220

Chicago/Turabian Style

Tang, Liqun, Huayu Xu, Yifeng Wang, Huimei Wang, Zhiyong Li, Xixi Liu, Yazhou Shu, Guan Li, Wanning Liu, Jiezheng Ying, and et al. 2021. "OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (Oryza sativa L.)" International Journal of Molecular Sciences 22, no. 22: 12220. https://doi.org/10.3390/ijms222212220

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop