The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway
Abstract
:1. Introduction
2. Results
2.1. Apigenin Hampers the Proliferation of Spermatogonia
2.2. Apigenin Promotes Apoptosis of Spermatogonia
2.3. Apigenin Reduces the Expression of Prmt7 and Akt3 in Spermatogonia
2.4. Prmt7 Is Abundantly Expressed in Mouse Testis
2.5. Prmt7 Knockdown Recapitulates the Inhibitory Effects of Apigenin on Spermatogonial Proliferation
2.6. Downregulation of Prmt7 Expression Goes beyond This Gene and Perturbs Several Other Genes
2.7. The Possible Functional Pathway Underlying the Regulation of Spermatogonial Proliferation
3. Discussion
4. Materials and Methods
4.1. Animals and Chemicals
4.2. Cell Culture and Apigenin Treatment
4.3. Cell Proliferation Assay
4.4. siRNA Transfection
4.5. Cell Cycle Analysis by Flow Cytometry
4.6. Cell Apoptosis Analysis by Flow Cytometry
4.7. RNA Extraction and RT-qPCR
4.8. Western Blotting
4.9. Library Preparation for RNA Sequencing
4.10. RNA Sequencing Data Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, B.; Zhang, M.; Guo, J.; Liu, Z.; Zhou, R.; Guo, F.; Li, K.; Mu, Y. The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway. Int. J. Mol. Sci. 2021, 22, 12209. https://doi.org/10.3390/ijms222212209
Wang B, Zhang M, Guo J, Liu Z, Zhou R, Guo F, Li K, Mu Y. The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway. International Journal of Molecular Sciences. 2021; 22(22):12209. https://doi.org/10.3390/ijms222212209
Chicago/Turabian StyleWang, Bingyuan, Mingrui Zhang, Jiankang Guo, Zhiguo Liu, Rong Zhou, Fei Guo, Kui Li, and Yulian Mu. 2021. "The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway" International Journal of Molecular Sciences 22, no. 22: 12209. https://doi.org/10.3390/ijms222212209