Distinct Mechanisms Account for In Vitro Activation and Sensitization of TRPV1 by the Porphyrin Hemin
Abstract
:1. Introduction
2. Results
2.1. Hemin Induces a Calcium Influx in Mouse DRG Neurons
2.2. Hemin Induces an Activation and Sensitization of hTRPV1 Expressed in HEK293t Cells
2.3. Sensitization of TRPV1 by Hemin Is Mediated by PKC
2.4. UVA-Light Triggers Direct Activation of TRPV1 and TRPA1 by Hemin
3. Discussion
4. Materials and Methods
4.1. Cell Culture and cDNA
4.2. Chemicals
4.3. Whole-Cell Patch Clamp
4.4. Calcium Imaging
4.5. Dorsal Root Ganglion Neuron Culture
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Palmaers, N.E.; Wiegand, S.B.; Herzog, C.; Echtermeyer, F.G.; Eberhardt, M.J.; Leffler, A. Distinct Mechanisms Account for In Vitro Activation and Sensitization of TRPV1 by the Porphyrin Hemin. Int. J. Mol. Sci. 2021, 22, 10856. https://doi.org/10.3390/ijms221910856
Palmaers NE, Wiegand SB, Herzog C, Echtermeyer FG, Eberhardt MJ, Leffler A. Distinct Mechanisms Account for In Vitro Activation and Sensitization of TRPV1 by the Porphyrin Hemin. International Journal of Molecular Sciences. 2021; 22(19):10856. https://doi.org/10.3390/ijms221910856
Chicago/Turabian StylePalmaers, Natalie E., Steffen B. Wiegand, Christine Herzog, Frank G. Echtermeyer, Mirjam J. Eberhardt, and Andreas Leffler. 2021. "Distinct Mechanisms Account for In Vitro Activation and Sensitization of TRPV1 by the Porphyrin Hemin" International Journal of Molecular Sciences 22, no. 19: 10856. https://doi.org/10.3390/ijms221910856