Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers
Abstract
:1. Introduction
2. Results
2.1. Isolation of Human Medullary Thyroid Cancer Glycosphingolipids
2.2. Characterization of the Acid Glycosphingolipids from Human Medullary Thyroid Cancer
2.3. Characterization of the Non-Acid Glycosphingolipids from Human Medullary Thyroid Cancer
2.3.1. LC-ESI/MS of Glycosphingolipid-Derived Oligosaccharides
α-N-Acetylgalactosaminidase Hydrolysis
2.3.2. Separation of the Non-Acid Glycosphingolipids
2.3.3. LC-ESI/MS of Fraction T-1
2.3.4. LC-ESI/MS of Fractions T2–T4
2.3.5. Binding of Carbohydrate Recognizing Ligands to the Non-Acid Glycosphingolipids
2.4. Immunohistochemistry
3. Discussion
4. Materials and Methods
4.1. Glycosphingolipid Preparations
4.2. Reference Glycosphingolipids
4.3. Thin-Layer Chromatography
4.4. Chromatogram Binding Assays
4.5. LC-ESI/MS of Native Acid Glycosphingolipids
4.6. Endoglycoceramidase Digestion and LC-ESI/MS
α-N-Acetylgalactosaminidase Hydrolysis
4.7. Histology and Immunohistochemistry
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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m/z | Trivial Name | Structure |
---|---|---|
794 | Sulfatide | SO3-3Galβ1Cer |
1151 | Neu5Ac-GM3 | Neu5Acα3Galβ4Glcβ1Cer |
721 | Neu5Ac-GD3 | Neu5Acα8Neu5Acα3Galβ4Glcβ1Cer |
959 | Neu5Ac-GD1a | Neu5Acα3Galβ3GalNAcβ4(Neu5Acα3)Galβ4Glcβ1Cer |
m/z | Trivial Name | Structure |
---|---|---|
503 | Globotri (Gb3) | Galα4Galβ4Glc |
706 | Neolactotetra (nLc4) | Galβ4GlcNAcβ3Galβ4Glc |
706 | Globotetra (Gb4) | GalNAcβ3Galα4Galβ4Glc |
909 | Forssman | GalNAcα3GalNAcβ3Galα4Galβ4Glc |
909 | x2 penta (x2) | GalNAcβ3Galβ4GlcNAcβ3Galβ4Glc |
852 | H type 2 penta (H5-2) | Fucα2Galβ4GlcNAcβ3Galβ4Glc |
852 | Lex penta (Lex-5) | Galβ4(Fucα3)GlcNAcβ3Galβ4Glc |
998 | Ley hexa (Ley-6) | Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glc |
1016 (red) * | Globo H | Fucα2Galβ3GalNAcβ3Galα4Galβ4Glc |
1055 | A hexa type 2 (A6-2) | GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glc |
1201 | A hepta type 2 (A7-2) | GalNAcα3(Fucα2)Galβ4(Fucα3)GlcNAcβ3Galβ4Glc |
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Säljö, K.; Thornell, A.; Jin, C.; Norlén, O.; Teneberg, S. Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers. Int. J. Mol. Sci. 2021, 22, 10463. https://doi.org/10.3390/ijms221910463
Säljö K, Thornell A, Jin C, Norlén O, Teneberg S. Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers. International Journal of Molecular Sciences. 2021; 22(19):10463. https://doi.org/10.3390/ijms221910463
Chicago/Turabian StyleSäljö, Karin, Anders Thornell, Chunsheng Jin, Olov Norlén, and Susann Teneberg. 2021. "Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers" International Journal of Molecular Sciences 22, no. 19: 10463. https://doi.org/10.3390/ijms221910463