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Article

Differential Regulation of NAPDH Oxidases in Salt-Tolerant Eutrema salsugineum and Salt-Sensitive Arabidopsis thaliana

1
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland
2
Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115 Bonn, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Mirza Hasanuzzaman and Masayuki Fujita
Int. J. Mol. Sci. 2021, 22(19), 10341; https://doi.org/10.3390/ijms221910341
Received: 29 August 2021 / Revised: 20 September 2021 / Accepted: 21 September 2021 / Published: 25 September 2021
Reactive oxygen species (ROS) signalling is crucial in modulating stress responses in plants, and NADPH oxidases (NOXs) are an important component of signal transduction under salt stress. The goal of this research was to investigate whether the regulation of NOX-dependent signalling during mild and severe salinity differs between the halophyte Eutrema salsugineum and the glycophyte Arabidopsis thaliana. Gene expression analyses showed that salt-induced expression patterns of two NOX genes, RBOHD and RBOHF, varied between the halophyte and the glycophyte. Five days of salinity stimulated the expression of both genes in E. salsugineum leaves, while their expression in A. thaliana decreased. This was not accompanied by changes in the total NOX activity in E. salsugineum, while the activity in A. thaliana was reduced. The expression of the RBOHD and RBOHF genes in E. salsugineum leaves was induced by abscisic acid (ABA) and ethephon spraying. The in silico analyses of promoter sequences of RBOHD and RBOHF revealed multiple cis-acting elements related to hormone responses, and their distribution varied between E. salsugineum and A. thaliana. Our results indicate that, in the halophyte E. salsugineum, the maintenance of the basal activity of NOXs in leaves plays a role during acclimation responses to salt stress. The different expression patterns of the RBOHD and RBOHF genes under salinity in E. salsugineum and A. thaliana point to a modified regulation of these genes in the halophyte, possibly through ABA- and/or ethylene-dependent pathways. View Full-Text
Keywords: halophyte species; NADPH oxidases; NOX; respiratory burst oxidase homolog RBOH gene expression; saline adaptations halophyte species; NADPH oxidases; NOX; respiratory burst oxidase homolog RBOH gene expression; saline adaptations
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MDPI and ACS Style

Pilarska, M.; Bartels, D.; Niewiadomska, E. Differential Regulation of NAPDH Oxidases in Salt-Tolerant Eutrema salsugineum and Salt-Sensitive Arabidopsis thaliana. Int. J. Mol. Sci. 2021, 22, 10341. https://doi.org/10.3390/ijms221910341

AMA Style

Pilarska M, Bartels D, Niewiadomska E. Differential Regulation of NAPDH Oxidases in Salt-Tolerant Eutrema salsugineum and Salt-Sensitive Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22(19):10341. https://doi.org/10.3390/ijms221910341

Chicago/Turabian Style

Pilarska, Maria, Dorothea Bartels, and Ewa Niewiadomska. 2021. "Differential Regulation of NAPDH Oxidases in Salt-Tolerant Eutrema salsugineum and Salt-Sensitive Arabidopsis thaliana" International Journal of Molecular Sciences 22, no. 19: 10341. https://doi.org/10.3390/ijms221910341

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