Integrated Metabolomic and Transcriptomic Analysis Reveals the Flavonoid Regulatory Network by Eutrema EsMYB90
Abstract
:1. Introduction
2. Results
2.1. Increased Flavonoid Compounds in EsMYB90 Transgenic Tobacco Leaves
2.2. Metabolite Analysis Based on OPLS-DA Model
2.3. Metabolic Profiling and Significantly Differential Metabolite Analysis
2.4. Integrated Analysis of Metabolite Profiling and RNA-seq in Phenylpropanoid/Flavonoid Biosynthesis Pathways
2.5. EsMYB90 Enhanced Flavonoid Metabolite Level via Activating Transcription of Flavonoid Biosynthesis Genes
3. Discussion
3.1. Key Role of EsMYB90 in Enhancing Antioxidative Metabolite Level
3.2. Regulating Mechanism of EsMYB90 in Phenylpropanoid/Flavonoid Pathways and Its Novelty
3.3. EsMYB90—A Potential Important Gene for Genetic Breeding to Improve the Flavonoid Level of Crops
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Measurement of Anthocyanin, Total Flavonoid, PA and Chlorophyll
4.3. RNA-Seq and Bioinformatic Analysis
4.4. Analysis of Metabolite Profiling
4.5. Differential Metabolite Analysis and Metabolic Pathways Construction
4.6. Integrated Analysis of Metabolome and Transcriptome
4.7. Dual Luciferase Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
4CL | 4-coumarate-CoA ligase |
ANR | Anthocyanidin reductase |
C4H | Trans-cinnamate 4-monooxygenase |
CCoAOMT | Caffeoyl-CoA O-methyltransferase |
CHI | Chalcone isomerase |
CHS | Chalcone synthase |
DEG | Differential expressed gene |
DFR | Dihydroflavonol reductase |
DHK | Dihydrokaempferol |
DHM | Dihydromyricetin |
DHQ | Dihydroquercetin |
EBGs | Early biosynthesis genes |
EGC | Epigallocatechin |
F3′5′H | Flavonoid 3′5′- hydroxylase |
F3′H | Flavonoid 3′-hydroxylase |
F3H | Flavanone 3-hydroxylase |
FLS | Flavonol synthase |
KEGG | Kyoto Encyclopedia of Genes and Genomes |
LAR | Leucoanthocyanidin reductase |
LBGs | Late biosynthetic genes |
LDOX/ANS | Leucoanthocyanidin dioxygenase/anthocyanidin synthase |
MBW | MYB-bHLH-WD40 |
ML | Mature leaves |
MS/MS | Tandem mass spectrometry |
MWDB | MetWare database |
MYB | v-myb avian myeloblastosis viral oncogene homolog |
OPLS-DA | Orthogonal partial least squares-discriminant analysis |
PA | Proanthocyanidin |
PAL | Phenylalanine ammonia lyase |
PCA | Principal component analysis |
PCC | Pearson correlation coefficient |
PCCP | p-value of Pearson correlation coefficient |
UFGT | UDP-glucose:flavonoid 3-glucosyltransferase |
UPLC | Ultra-performance liquid chromatography |
VIP | Variable importance in projection |
YL | Young leaves |
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Qi, Y.; Li, C.; Duan, C.; Gu, C.; Zhang, Q. Integrated Metabolomic and Transcriptomic Analysis Reveals the Flavonoid Regulatory Network by Eutrema EsMYB90. Int. J. Mol. Sci. 2021, 22, 8751. https://doi.org/10.3390/ijms22168751
Qi Y, Li C, Duan C, Gu C, Zhang Q. Integrated Metabolomic and Transcriptomic Analysis Reveals the Flavonoid Regulatory Network by Eutrema EsMYB90. International Journal of Molecular Sciences. 2021; 22(16):8751. https://doi.org/10.3390/ijms22168751
Chicago/Turabian StyleQi, Yuting, Chuanshun Li, Chonghao Duan, Caihong Gu, and Quan Zhang. 2021. "Integrated Metabolomic and Transcriptomic Analysis Reveals the Flavonoid Regulatory Network by Eutrema EsMYB90" International Journal of Molecular Sciences 22, no. 16: 8751. https://doi.org/10.3390/ijms22168751