Chronic and Intermittent Hyperglycemia Modulates Expression of Key Molecules of PI3K/AKT Pathway in Differentiating Human Visceral Adipocytes
Abstract
:1. Introduction
2. Results
2.1. Evaluation of the Gene Expression Profile of Insulin Signaling Pathway Molecules under CHG and IHG
2.2. Evaluation of Insulin Signaling Proteins Expression during ADG in CHG and IHG
2.3. Expression of Selected miRs That Target Insulin Signaling Pathway Molecules during ADG in CHG and IHG
3. Discussion
3.1. ADG in CHG
3.2. ADG in IHG
3.3. Study Limitations
3.4. Overall Conclusions and Clinical Output
4. Materials and Methods
4.1. Cell Culture
4.2. RNA Isolation and mRNA Expression Profiling
4.3. Protein Isolation and Expression Profiling
4.4. miRs Isolation and Expression Profiling
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ADG | adipogenesis |
Ads | adipocytes |
AKT | protein kinase B |
AT | adipose tissue |
CHG | chronic hyperglycemia |
C/EBPs | CCAAT enhancer-binding proteins |
FOXO1 | forkhead box O1 |
GLUT4 | glucose transporter 4 |
HG | hyperglycemia |
IHG | intermittent hyperglycemia |
mTOR2 | mammalian target of rapamycin 2 |
NG | normoglycemia |
pAds | preadipocytes |
PDK1 | phosphoinositide-dependent kinase 1 |
PIP2 | phophatidylinositol-4,5-biphospate |
PIP3 | phophatidylinositol-3,4,5-triphospate |
PI3K | phosphatidylinositol-4,5-biphosphate 3-kinase |
PI3K-C | phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit |
PI3K-R | phosphatidylinositol-4,5-biphosphate 3-kinase regulatory subunit |
PPARG | peroxisome proliferator-activated receptor gamma |
PTEN | phosphatase and tensin homolog |
T2DM | type 2 diabetes mellitus |
VAT | visceral adipose tissue |
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Mir | Target Genes | References |
---|---|---|
29a-3p | IRS, AKT, PI3KR, PTEN, GLUT4 | [18,20,21,22,23,24,25,26,27,28] |
143-3p | AKT, GLUT4 | [18] |
152-3p | IRS, PTEN | [29,30] |
186-5p | PI3KR, PTEN | [31,32] |
370-3p | IRS | [33] |
374b-5p | IRS, PI3KC, PTEN | [34,35,36,37] |
Proliferation | Differentiation | Maturation |
---|---|---|
N | - | - |
H | - | - |
N | N | - |
N | H | - |
H | N | - |
H | H | - |
N | N | N |
N | N | H |
N | H | H |
N | H | N |
H | H | H |
H | H | N |
H | N | H |
H | N | N |
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Świderska, E.; Strycharz, J.; Wróblewski, A.; Czarny, P.; Szemraj, J.; Drzewoski, J.; Śliwińska, A. Chronic and Intermittent Hyperglycemia Modulates Expression of Key Molecules of PI3K/AKT Pathway in Differentiating Human Visceral Adipocytes. Int. J. Mol. Sci. 2021, 22, 7712. https://doi.org/10.3390/ijms22147712
Świderska E, Strycharz J, Wróblewski A, Czarny P, Szemraj J, Drzewoski J, Śliwińska A. Chronic and Intermittent Hyperglycemia Modulates Expression of Key Molecules of PI3K/AKT Pathway in Differentiating Human Visceral Adipocytes. International Journal of Molecular Sciences. 2021; 22(14):7712. https://doi.org/10.3390/ijms22147712
Chicago/Turabian StyleŚwiderska, Ewa, Justyna Strycharz, Adam Wróblewski, Piotr Czarny, Janusz Szemraj, Józef Drzewoski, and Agnieszka Śliwińska. 2021. "Chronic and Intermittent Hyperglycemia Modulates Expression of Key Molecules of PI3K/AKT Pathway in Differentiating Human Visceral Adipocytes" International Journal of Molecular Sciences 22, no. 14: 7712. https://doi.org/10.3390/ijms22147712