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Article

Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester

1
School of Chemistry, Institute of Science, Center for Biomolecular Structure, Function and Application, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
2
Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Luigi Cattivelli
Int. J. Mol. Sci. 2021, 22(14), 7593; https://doi.org/10.3390/ijms22147593
Received: 20 April 2021 / Revised: 11 July 2021 / Accepted: 12 July 2021 / Published: 15 July 2021
(This article belongs to the Section Molecular Plant Sciences)
Conjugation of phytohormones with glucose is a means of modulating their activities, which can be rapidly reversed by the action of β-glucosidases. Evaluation of previously characterized recombinant rice β-glucosidases found that nearly all could hydrolyze abscisic acid glucose ester (ABA-GE). Os4BGlu12 and Os4BGlu13, which are known to act on other phytohormones, had the highest activity. We expressed Os4BGlu12, Os4BGlu13 and other members of a highly similar rice chromosome 4 gene cluster (Os4BGlu9, Os4BGlu10 and Os4BGlu11) in transgenic Arabidopsis. Extracts of transgenic lines expressing each of the five genes had higher β-glucosidase activities on ABA-GE and gibberellin A4 glucose ester (GA4-GE). The β-glucosidase expression lines exhibited longer root and shoot lengths than control plants in response to salt and drought stress. Fusions of each of these proteins with green fluorescent protein localized near the plasma membrane and in the apoplast in tobacco leaf epithelial cells. The action of these extracellular β-glucosidases on multiple phytohormones suggests they may modulate the interactions between these phytohormones. View Full-Text
Keywords: abscisic acid; β-Glucosidase; glycosylation; Oryza sativa; phytohormone conjugates abscisic acid; β-Glucosidase; glycosylation; Oryza sativa; phytohormone conjugates
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MDPI and ACS Style

Kongdin, M.; Mahong, B.; Lee, S.-K.; Shim, S.-H.; Jeon, J.-S.; Ketudat Cairns, J.R. Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester. Int. J. Mol. Sci. 2021, 22, 7593. https://doi.org/10.3390/ijms22147593

AMA Style

Kongdin M, Mahong B, Lee S-K, Shim S-H, Jeon J-S, Ketudat Cairns JR. Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester. International Journal of Molecular Sciences. 2021; 22(14):7593. https://doi.org/10.3390/ijms22147593

Chicago/Turabian Style

Kongdin, Manatchanok, Bancha Mahong, Sang-Kyu Lee, Su-Hyeon Shim, Jong-Seong Jeon, and James R. Ketudat Cairns. 2021. "Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester" International Journal of Molecular Sciences 22, no. 14: 7593. https://doi.org/10.3390/ijms22147593

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