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Article

Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone

1
Centro de Biomateriales, Universidad de La Habana, La Habana 10400, Cuba
2
TNI Group, Department of Radiology, Leiden University Medical Center (LUMC), 2333 ZA Leiden, The Netherlands
3
Laboratorio de Biología Molecular, Departamento de Radiobiología, Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), La Habana 11300, Cuba
4
Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Posgrado, Universidad Católica del Maule, Talca 3460000, Chile
5
Department of Chemical Engineering, Universidad de San Francisco de Quito, Quito 170901, Ecuador
*
Author to whom correspondence should be addressed.
Academic Editor: Peter John Jervis
Int. J. Mol. Sci. 2021, 22(11), 5730; https://doi.org/10.3390/ijms22115730
Received: 31 March 2021 / Revised: 8 May 2021 / Accepted: 11 May 2021 / Published: 27 May 2021
(This article belongs to the Special Issue Hydrogels in Regenerative Medicine and Other Biomedical Applications)
Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead® assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility. View Full-Text
Keywords: hydrogels; sodium alginate; betamethasone; drug delivery; cartilage tissue engineering hydrogels; sodium alginate; betamethasone; drug delivery; cartilage tissue engineering
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MDPI and ACS Style

García-Couce, J.; Vernhes, M.; Bada, N.; Agüero, L.; Valdés, O.; Alvarez-Barreto, J.; Fuentes, G.; Almirall, A.; Cruz, L.J. Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone. Int. J. Mol. Sci. 2021, 22, 5730. https://doi.org/10.3390/ijms22115730

AMA Style

García-Couce J, Vernhes M, Bada N, Agüero L, Valdés O, Alvarez-Barreto J, Fuentes G, Almirall A, Cruz LJ. Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone. International Journal of Molecular Sciences. 2021; 22(11):5730. https://doi.org/10.3390/ijms22115730

Chicago/Turabian Style

García-Couce, Jomarien, Marioly Vernhes, Nancy Bada, Lissette Agüero, Oscar Valdés, José Alvarez-Barreto, Gastón Fuentes, Amisel Almirall, and Luis J. Cruz 2021. "Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone" International Journal of Molecular Sciences 22, no. 11: 5730. https://doi.org/10.3390/ijms22115730

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