Next Article in Journal
Robust Identification of Suitable T-Cell Subsets for Personalized CMV-Specific T-Cell Immunotherapy Using CD45RA and CD62L Microbeads
Previous Article in Journal
Periodontal Disease: A Risk Factor for Diabetes and Cardiovascular Disease
Open AccessArticle

Functional Mechanisms Underlying the Antimicrobial Activity of the Oryza sativa Trx-like Protein

1
Department of Polymer Science and Engineering, Sunchon National University, Suncheon 57922, Korea
2
National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 33657, Korea
3
Division of Applied Life Science (BK21+ Program) and PMBBRC, Gyeongsang National University, Jinju 52828, Korea
4
Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843, USA
5
The Research Institute for Sanitation and Environment of Coastal Areas, Sunchon National University, Suncheon 57922, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(6), 1413; https://doi.org/10.3390/ijms20061413
Received: 31 January 2019 / Revised: 13 March 2019 / Accepted: 19 March 2019 / Published: 20 March 2019
(This article belongs to the Section Molecular Plant Sciences)
Plants are constantly subjected to a variety of environmental stresses and have evolved regulatory responses to overcome unfavorable conditions that might reduce or adversely change a plant’s growth or development. Among these, the regulated production of reactive oxygen species (ROS) as a signaling molecule occurs during plant development and pathogen defense. This study demonstrates the possible antifungal activity of Oryza sativa Tetratricopeptide Domain-containing thioredoxin (OsTDX) protein against various fungal pathogens. The transcription of OsTDX was induced by various environmental stresses known to elicit the generation of ROS in plant cells. OsTDX protein showed potent antifungal activity, with minimum inhibitory concentrations (MICs) against yeast and filamentous fungi ranging between 1.56 and 6.25 and 50 and 100 µg/mL, respectively. The uptake of SYTOX-Green into fungal cells and efflux of calcein from artificial fungus-like liposomes suggest that its killing mechanism involves membrane permeabilization and damage. In addition, irregular blebs and holes apparent on the surfaces of OsTDX-treated fungal cells indicate the membranolytic action of this protein. Our results suggest that the OsTDX protein represents a potentially useful lead for the development of pathogen-resistant plants. View Full-Text
Keywords: antifungal activity; plant defense; reactive oxygen species; thioredoxin; tetratricopeptide repeat domain antifungal activity; plant defense; reactive oxygen species; thioredoxin; tetratricopeptide repeat domain
Show Figures

Figure 1

MDPI and ACS Style

Park, S.-C.; Kim, I.R.; Hwang, J.E.; Kim, J.-Y.; Jung, Y.J.; Choi, W.; Lee, Y.; Jang, M.-K.; Lee, J.R. Functional Mechanisms Underlying the Antimicrobial Activity of the Oryza sativa Trx-like Protein. Int. J. Mol. Sci. 2019, 20, 1413. https://doi.org/10.3390/ijms20061413

AMA Style

Park S-C, Kim IR, Hwang JE, Kim J-Y, Jung YJ, Choi W, Lee Y, Jang M-K, Lee JR. Functional Mechanisms Underlying the Antimicrobial Activity of the Oryza sativa Trx-like Protein. International Journal of Molecular Sciences. 2019; 20(6):1413. https://doi.org/10.3390/ijms20061413

Chicago/Turabian Style

Park, Seong-Cheol; Kim, Il R.; Hwang, Jung E.; Kim, Jin-Young; Jung, Young J.; Choi, Wonkyun; Lee, Yongjae; Jang, Mi-Kyeong; Lee, Jung R. 2019. "Functional Mechanisms Underlying the Antimicrobial Activity of the Oryza sativa Trx-like Protein" Int. J. Mol. Sci. 20, no. 6: 1413. https://doi.org/10.3390/ijms20061413

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop