Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
Abstract
:1. Introduction
2. Results
2.1. The Wnt Gene Repertoire in the Silkworm
2.2. Phylogenetic Analysis of BmWnts
2.3. The Conserved Wnt Gene Cluster
2.4. Embryonic Expression Profile of the BmWnt Genes
2.5. Different Tissue Expression Profiles of the BmWnt Genes
3. Discussion
4. Materials and Methods
4.1. Genome-Wide Identification of Wnt Proteins
4.2. Chromosomal Distribution and Protein Structure
4.3. Sequence Alignment and Phylogenetic Analysis
4.4. RNA Extraction and cDNA Synthesis
4.5. Real-Time Quantitative PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PCP | planar cell polarity |
Fzd | Frizzled |
LEF1/TCF | T-cell factor/lymphoid enhancing factor |
JNK | Jun N-terminal serine/threonine kinase |
Rock | Rho-associated kinase |
CamKI | Ca2+–calmodulin-dependent protein kinase II |
PKC | protein kinase C |
HMM | Hidden Markov Model |
SMART | Simple Modular Architecture Research Tool |
AEL | after egg laying |
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GeneName | GeneID | Scaff_ID | Probe ID |
---|---|---|---|
BGIBMGA006146 | BmWnt1 | nscaf2847: 4290086..4300264: + | sw07406 |
BGIBMGA013783 | BmWnt5 | nscaf3097: 574395..598709: + | sw10651 |
BGIBMGA006004 | BmWnt6 | nscaf2847: 4311541..4316713: − | sw10550 |
BGIBMGA013981 | BmWnt7 | nscaf3099: 2430055..2453230: + | none |
BGIBMGA006145 | BmWnt9 | nscaf2847: 4227852..4228750: + | sw17884 |
BGIBMGA006147 BGIBMGA006148 | BmWnt10 | nscaf2847: 4406381..4410443: + | sw21982 |
BGIBMGA007580 | BmWnt11 | nscaf2887: 11667287..1177617: + | sw15093 |
BGIBMGA013787 | BmWntA | nscaf3097: 771657..781653: + | sw13710 |
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Ding, X.; Liu, J.; Zheng, L.; Song, J.; Li, N.; Hu, H.; Tong, X.; Dai, F. Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori. Int. J. Mol. Sci. 2019, 20, 1221. https://doi.org/10.3390/ijms20051221
Ding X, Liu J, Zheng L, Song J, Li N, Hu H, Tong X, Dai F. Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori. International Journal of Molecular Sciences. 2019; 20(5):1221. https://doi.org/10.3390/ijms20051221
Chicago/Turabian StyleDing, Xin, Junxia Liu, Lu Zheng, Jiangbo Song, Niannian Li, Hai Hu, Xiaoling Tong, and Fangyin Dai. 2019. "Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori" International Journal of Molecular Sciences 20, no. 5: 1221. https://doi.org/10.3390/ijms20051221