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Open AccessArticle

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

1
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
2
Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China
3
Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China
4
Guangdong Provincial Fishery Germplasm Conservation Center, Guangzhou 511400, China
5
Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230000, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5602; https://doi.org/10.3390/ijms20225602 (registering DOI)
Received: 13 September 2019 / Revised: 1 November 2019 / Accepted: 6 November 2019 / Published: 9 November 2019
(This article belongs to the Section Molecular Biology)
Hepcidin is a liver-derived peptide hormone that is related to iron balance and immunity in humans. However, its function in Siniperca chuatsi has not been well elucidated. In this study, we analyzed the expression and function of the S. chuatsi hepcidin (Sc-hep) gene. Sc-hep was specifically expressed in the liver and appeared to be one of the most highly expressed genes in the liver. After spleen and kidney necrosis virus (ISKNV) infection and lipopolysaccharide (LPS) and polyinosinic—polycytidylic acid (Poly I:C) stimulation, the expression of Sc-hep in the liver increased by approximately 110, 6500, and 225 times, respectively. After ferrous sulfate (FS) injection, the expression of Sc-hep in the liver increased approximately 520-fold. We found that miR-19c-5p could inhibit Sc-hep expression. Five CpG dinucleotides distributed in the promoter region showed no differential methylation between the liver and the stomach, both presenting high methylation rates. After FS or LPS injection, the expression of three iron balance-related genes (FPN1, TFR1, and FTN) and five immune-related cytokine genes (IL-1β, IL8, TNF-α, TLR22, and SOCS3) significantly changed. These results indicate that Sc-hep participates in the regulation of iron balance and plays an important role in the immune system. Sc-hep increased approximately 52-fold when mandarin fish were domesticated with artificial diets. Sc-hep might be used as a real-time biomarker of mandarin fish liver because its expression markedly varies under different physiological conditions. View Full-Text
Keywords: mandarin fish; liver; hepcidin; iron balance; immune response; miR-19c-5p mandarin fish; liver; hepcidin; iron balance; immune response; miR-19c-5p
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Shen, Y.; Zhao, Z.; Zhao, J.; Chen, X.; Cao, M.; Wu, M. Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi). Int. J. Mol. Sci. 2019, 20, 5602.

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