Next Article in Journal
RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes
Previous Article in Journal
Plant-Derived Inhibitors of AHL-Mediated Quorum Sensing in Bacteria: Modes of Action
Open AccessArticle

Enhanced Therapeutic Potential of the Secretome Released from Adipose-Derived Stem Cells by PGC-1α-Driven Upregulation of Mitochondrial Proliferation

1
Department of Surgery, Uijeongbu St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Seoul 11765, Korea
2
Department of Surgery, Seoul St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Seoul 06591, Korea
3
Catholic Central Laboratory of Surgery, College of Medicine, the Catholic University of Korea, Seoul 06591, Korea
4
Department of Surgery, Daejeon St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Seoul 34943, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5589; https://doi.org/10.3390/ijms20225589
Received: 16 October 2019 / Revised: 5 November 2019 / Accepted: 7 November 2019 / Published: 8 November 2019
(This article belongs to the Section Molecular Biology)
Peroxisome proliferator activated receptor λ coactivator 1α (PGC-1α) is a potent regulator of mitochondrial biogenesis and energy metabolism. In this study, we investigated the therapeutic potential of the secretome released from the adipose-derived stem cells (ASCs) transfected with PGC-1α (PGC-secretome). We first generated PGC-1α-overexpressing ASCs by transfecting ASCs with the plasmids harboring the gene encoding PGC-1α. Secretory materials released from PGC-1α-overexpressing ASCs were collected and their therapeutic potential was determined using in vitro (thioacetamide (TAA)-treated AML12 cells) and in vivo (70% partial hepatectomized mice) models of liver injury. In the TAA-treated AML12 cells, the PGC-secretome significantly increased cell viability, promoted expression of proliferation-related markers, such as PCNA and p-STAT, and significantly reduced the levels of reactive oxygen species (ROS). In the mice, PGC-secretome injections significantly increased liver tissue expression of proliferation-related markers more than normal secretome injections did (p < 0.05). We demonstrated that the PGC-secretome does not only have higher antioxidant and anti-inflammatory properties, but also has the potential of significantly enhancing liver regeneration in both in vivo and in vitro models of liver injury. Thus, reinforcing the mitochondrial antioxidant potential by transfecting ASCs with PGC-1α could be one of the effective strategies to enhance the therapeutic potential of ASCs. View Full-Text
Keywords: adipose-derived stem cell; liver regeneration; reactive oxygen species; peroxisome proliferator activated receptor λ coactivator 1α (PGC-1α); secretome adipose-derived stem cell; liver regeneration; reactive oxygen species; peroxisome proliferator activated receptor λ coactivator 1α (PGC-1α); secretome
Show Figures

Figure 1

MDPI and ACS Style

Lee, J.; Kim, O.-H.; Lee, S.C.; Kim, K.-H.; Shin, J.S.; Hong, H.-E.; Choi, H.J.; Kim, S.-J. Enhanced Therapeutic Potential of the Secretome Released from Adipose-Derived Stem Cells by PGC-1α-Driven Upregulation of Mitochondrial Proliferation. Int. J. Mol. Sci. 2019, 20, 5589.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop