Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats
Abstract
:1. Introduction
2. Results
2.1. MgSO4 Improves Survival in Monocrotaline-Treated Rats
2.2. MgSO4 Mitigates Monocrotaline-Induced Pulmonary Hypertension
2.3. MgSO4 Mitigates Monocrotaline-Induced Pulmonary Artery Remodeling, Lung Tissue Lipid Peroxidation, and Pulmonary Artery Mitochondrial Injury
2.4. MgSO4 Mitigates Monocrotaline-Induced Inflammation and Apoptosis in Lung Tissues
2.5. MgSO4 Mitigates Monocrotaline-Induced Right Ventricular Hypertrophy
2.6. MgSO4 Mitigates Monocrotaline-Induced Lipid Peroxidation, Mitochondrial Injury, Inflammation, and Apoptosis in Myocardium
2.7. Calcium Chloride Counteracts the Effect of Mgso4 in Monocrotaline-Treated Rats
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Monocrotaline Pulmonary Hypertension Model and Dosages of Monocrotaline and MgSO4
4.3. Study Protocols
4.4. RVSP and RV/(LV+S) Ratio Measurements
4.5. Assay of Wall Thickness of Pulmonary Arteries
4.6. Immunofluorescence Staining
4.7. Immunoblotting Assay
4.8. Enzyme-linked Immunosorbent Assay
4.9. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling
4.10. Immunohistochemistry Staining Assay
4.11. Masson’s Trichrome Staining
4.12. Transmission Electron Microscopic Analysis
4.13. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Chang, C.-Y.; Shih, H.-J.; Huang, I.-T.; Tsai, P.-S.; Chen, K.-Y.; Huang, C.-J. Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats. Int. J. Mol. Sci. 2019, 20, 4622. https://doi.org/10.3390/ijms20184622
Chang C-Y, Shih H-J, Huang I-T, Tsai P-S, Chen K-Y, Huang C-J. Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats. International Journal of Molecular Sciences. 2019; 20(18):4622. https://doi.org/10.3390/ijms20184622
Chicago/Turabian StyleChang, Chao-Yuan, Hung-Jen Shih, I-Tao Huang, Pei-Shan Tsai, Kung-Yen Chen, and Chun-Jen Huang. 2019. "Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats" International Journal of Molecular Sciences 20, no. 18: 4622. https://doi.org/10.3390/ijms20184622