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Open AccessArticle

OsCAF1, a CRM Domain Containing Protein, Influences Chloroplast Development

1
State Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China
2
Biotechnology Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Int. J. Mol. Sci. 2019, 20(18), 4386; https://doi.org/10.3390/ijms20184386
Received: 6 July 2019 / Revised: 30 August 2019 / Accepted: 3 September 2019 / Published: 6 September 2019
(This article belongs to the Special Issue Molecular Research in Rice: Agronomically Important Traits)
The chloroplast RNA splicing and ribosome maturation (CRM) domain proteins are involved in the splicing of chloroplast gene introns. Numerous CRM domain proteins have been reported to play key roles in chloroplast development in several plant species. However, the functions of CRM domain proteins in chloroplast development in rice remain poorly understood. In the study, we generated oscaf1 albino mutants, which eventually died at the seedling stage, through the editing of OsCAF1 with two CRM domains using CRISPR/Cas9 technology. The mesophyll cells in oscaf1 mutant had decreased chloroplast numbers and damaged chloroplast structures. OsCAF1 was located in the chloroplast, and transcripts revealed high levels in green tissues. In addition, the OsCAF1 promoted the splicing of group IIA and group IIB introns, unlike orthologous proteins of AtCAF1 and ZmCAF1, which only affected the splicing of subgroup IIB introns. We also observed that the C-terminal of OsCAF1 interacts with OsCRS2, and OsCAF1–OsCRS2 complex may participate in the splicing of group IIA and group IIB introns in rice chloroplasts. OsCAF1 regulates chloroplast development by influencing the splicing of group II introns. View Full-Text
Keywords: chloroplast RNA splicing and ribosome maturation (CRM) domain; intron splicing; chloroplast development; rice chloroplast RNA splicing and ribosome maturation (CRM) domain; intron splicing; chloroplast development; rice
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Zhang, Q.; Shen, L.; Wang, Z.; Hu, G.; Ren, D.; Hu, J.; Zhu, L.; Gao, Z.; Zhang, G.; Guo, L.; Zeng, D.; Qian, Q. OsCAF1, a CRM Domain Containing Protein, Influences Chloroplast Development. Int. J. Mol. Sci. 2019, 20, 4386.

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