Absence of MCP-induced Protein 1 Enhances Blood–Brain Barrier Breakdown after Experimental Stroke in Mice
Abstract
:1. Introduction
2. Results
2.1. MCPIP1 Is Up-Regulated in the Murine Brain Subjected to Transient Focal Ischemia/Reperfusion (I/R) Injury
2.2. Extravasation of FITC-Dextran Is Markedly Increased in the Brains of MCPIP1–/– Mice Subjected to Transient Focal I/R Injury
2.3. Matrix Metalloproteinase Expression in MCPIP1–/–Mice Subjected to Transient Focal I/R Injury
2.4. Altered Expression of Tight Junctions in MCPIP1−/− Mice Subjected to Transient Focal I/R Injury
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Transient Focal Brain Ischemia/Reperfusion in Mice
4.3. Assessment of BBB Breakdown Using FITC-Dextran
4.4. Quantitative Real-Time PCR
4.5. Western Blots
4.6. Immunohistochemistry
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
I/R | ischemia/reperfusion |
MCAO | middle cerebral artery occlusion |
MMPs | matrix metalloproteinases |
BBB | blood–brain barrier |
MCPIP1 | monocyte chemotactic protein 1–induced protein 1 |
FITC | fluorescein isothiocyanate |
ZO-1 | zonula occludens-1 |
MCP-1 | monocyte chemoattractant protein-1 |
TNF-α | tumor necrosis factor-alpha |
IL-1β | interleukin 1 beta |
IL-6 | interleukin 6 |
LPS | lipopolysaccharide |
qRT-PCR | quantitative real time PCR |
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Jin, Z.; Liang, J.; Li, J.; Kolattukudy, P.E. Absence of MCP-induced Protein 1 Enhances Blood–Brain Barrier Breakdown after Experimental Stroke in Mice. Int. J. Mol. Sci. 2019, 20, 3214. https://doi.org/10.3390/ijms20133214
Jin Z, Liang J, Li J, Kolattukudy PE. Absence of MCP-induced Protein 1 Enhances Blood–Brain Barrier Breakdown after Experimental Stroke in Mice. International Journal of Molecular Sciences. 2019; 20(13):3214. https://doi.org/10.3390/ijms20133214
Chicago/Turabian StyleJin, Zhuqing, Jian Liang, Jiaqi Li, and Pappachan E. Kolattukudy. 2019. "Absence of MCP-induced Protein 1 Enhances Blood–Brain Barrier Breakdown after Experimental Stroke in Mice" International Journal of Molecular Sciences 20, no. 13: 3214. https://doi.org/10.3390/ijms20133214