Next Article in Journal
HMGA2 Contributes to Distant Metastasis and Poor Prognosis by Promoting Angiogenesis in Oral Squamous Cell Carcinoma
Next Article in Special Issue
Genome Editing to Integrate Seed Size and Abiotic Stress Tolerance Traits in Arabidopsis Reveals a Role for DPA4 and SOD7 in the Regulation of Inflorescence Architecture
Previous Article in Journal
Transcriptomic Analysis of Leaf Sheath Maturation in Maize
Previous Article in Special Issue
Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.
Open AccessArticle

Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9

1
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(10), 2471; https://doi.org/10.3390/ijms20102471
Received: 10 April 2019 / Revised: 6 May 2019 / Accepted: 13 May 2019 / Published: 19 May 2019
(This article belongs to the Special Issue Genome Editing in Plants)
Cowpea (Vigna unguiculata) is widely cultivated across the world. Due to its symbiotic nitrogen fixation capability and many agronomically important traits, such as tolerance to low rainfall and low fertilization requirements, as well as its high nutrition and health benefits, cowpea is an important legume crop, especially in many semi-arid countries. However, research in Vigna unguiculata is dramatically hampered by the lack of mutant resources and efficient tools for gene inactivation in vivo. In this study, we used clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9). We applied the CRISPR/Cas9-mediated genome editing technology to efficiently disrupt the representative symbiotic nitrogen fixation (SNF) gene in Vigna unguiculata. Our customized guide RNAs (gRNAs) targeting symbiosis receptor-like kinase (SYMRK) achieved ~67% mutagenic efficiency in hairy-root-transformed plants, and nodule formation was completely blocked in the mutants with both alleles disrupted. Various types of mutations were observed near the PAM region of the respective gRNA. These results demonstrate the applicability of the CRISPR/Cas9 system in Vigna unguiculata, and therefore should significantly stimulate functional genomics analyses of many important agronomical traits in this unique crop legume. View Full-Text
Keywords: genome editing; CRISPR/Cas9; cowpea; symbiosis; nitrogen fixation genome editing; CRISPR/Cas9; cowpea; symbiosis; nitrogen fixation
Show Figures

Figure 1

MDPI and ACS Style

Ji, J.; Zhang, C.; Sun, Z.; Wang, L.; Duanmu, D.; Fan, Q. Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9. Int. J. Mol. Sci. 2019, 20, 2471.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop