5-Azacitidine Induces Cell Death in a Tissue Culture of Brachypodium distachyon
AbstractMorphological and histological observations revealed that, at a concentration of 50 µM, 5-azacitidine (5-azaC) totally inhibited the induction of embryogenic masses (EM), while the cultivation of explants (zygotic embryos; ZEs) in the presence of 5 µM of 5-azaC led to the formation of a callus with EM in 10% of the cases. Transmission electron microscopy (TEM) analyzes revealed the presence of the morphological and ultrastructural features that are typical for the vacuolar type of cell death in the callus cells that were treated. A TUNEL assay confirmed the presence of DNA double-strand breaks for the callus cells that had been treated with both 5 and 50 µM 5-azaC concentrations. Analysis of the gene expression of selected cell death markers demonstrated a reduced expression of metacaspase, protein executer 1 (EX1), and thioredoxin (TRX) in the callus cells that had been treated compared to the control culture. The strongest increase in the gene activity was characteristic for glutathione S-transferase (GST). Our studies also included an analysis of the distribution of some arabinogalactan proteins (AGPs) and extensin epitopes, which can be used as markers of cells that are undergoing death in a Brachypodium distachyon tissue culture. View Full-Text
Share & Cite This Article
Betekhtin, A.; Milewska-Hendel, A.; Chajec, L.; Rojek, M.; Nowak, K.; Kwasniewska, J.; Wolny, E.; Kurczynska, E.; Hasterok, R. 5-Azacitidine Induces Cell Death in a Tissue Culture of Brachypodium distachyon. Int. J. Mol. Sci. 2018, 19, 1806.
Betekhtin A, Milewska-Hendel A, Chajec L, Rojek M, Nowak K, Kwasniewska J, Wolny E, Kurczynska E, Hasterok R. 5-Azacitidine Induces Cell Death in a Tissue Culture of Brachypodium distachyon. International Journal of Molecular Sciences. 2018; 19(6):1806.Chicago/Turabian Style
Betekhtin, Alexander; Milewska-Hendel, Anna; Chajec, Lukasz; Rojek, Magdalena; Nowak, Katarzyna; Kwasniewska, Jolanta; Wolny, Elzbieta; Kurczynska, Ewa; Hasterok, Robert. 2018. "5-Azacitidine Induces Cell Death in a Tissue Culture of Brachypodium distachyon." Int. J. Mol. Sci. 19, no. 6: 1806.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.