Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation
by
Aya Mizutani Akimoto 1,*,†
, Erika Hasuike Niitsu 2,†, Kenichi Nagase 3,‡, Teruo Okano 3, Hideko Kanazawa 2 and Ryo Yoshida 1,*
Aya Mizutani Akimoto 1,*,†, Erika Hasuike Niitsu 2,†, Kenichi Nagase 3,‡, Teruo Okano 3, Hideko Kanazawa 2 and Ryo Yoshida 1,*
1
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan
2
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan
3
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
Present address: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.
Int. J. Mol. Sci. 2018, 19(4), 1253; https://doi.org/10.3390/ijms19041253
Received: 22 March 2018 / Revised: 11 April 2018 / Accepted: 18 April 2018 / Published: 21 April 2018
(This article belongs to the Special Issue Smart Polymers for Biomedical Applications)
We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa-arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow-derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post-crosslinking gelation method was revealed to be cytocompatible and showed temperature-dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.
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Keywords:
poly(N-isopropylacrylamide); hydrogel; mesenchymal stem cell; cell culture; mechanobiology
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MDPI and ACS Style
Akimoto, A.M.; Niitsu, E.H.; Nagase, K.; Okano, T.; Kanazawa, H.; Yoshida, R. Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation. Int. J. Mol. Sci. 2018, 19, 1253.
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