Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa
AbstractWe question whether the expression of GalNAc-T3, the only known O-GalNAc-transferase present in germ cells, is correlated with qualitative and functional parameters of spermatozoa. We investigated the expression of GalNAc-T3 in ejaculated spermatozoa with immunocytochemistry in swim-up purified and acrosome-reacted spermatozoa from quality-control semen donors and in semen samples from 206 randomly selected men representing a broad spectrum of semen quality. Using donor ejaculates and immunofluorescence detection we found that expression of GalNAc-T3 and the presence of the immature O-glycans Tn and T localized to the equatorial segment of spermatozoa. The proportion of GalNAc-T3-positive spermatozoa in the ejaculate increased after swim-up and appeared unaffected by induction of acrosomal exocytosis. The fraction of spermatozoa with equatorial expression of GalNAc-T3 correlated with classical semen parameters (concentration p = 9 × 10−6, morphology p = 7 × 10−8, and motility p = 1.8 × 10−5) and was significantly lower in men with oligoteratoasthenozoospermia (p = 0.0048). In conclusion, GalNAc-T3 was highly expressed by motile spermatozoa and the expression correlated positively with the classical semen parameters. Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O-glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa. View Full-Text
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Nygaard, M.B.; Herlihy, A.S.; Jeanneau, C.; Nielsen, J.E.; Bennett, E.P.; Jørgensen, N.; Clausen, H.; Mandel, U.; Rajpert-De Meyts, E.; Almstrup, K. Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa. Int. J. Mol. Sci. 2018, 19, 2949.
Nygaard MB, Herlihy AS, Jeanneau C, Nielsen JE, Bennett EP, Jørgensen N, Clausen H, Mandel U, Rajpert-De Meyts E, Almstrup K. Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa. International Journal of Molecular Sciences. 2018; 19(10):2949.Chicago/Turabian Style
Nygaard, Marie B.; Herlihy, Amy S.; Jeanneau, Charlotte; Nielsen, John E.; Bennett, Eric P.; Jørgensen, Niels; Clausen, Henrik; Mandel, Ulla; Rajpert-De Meyts, Ewa; Almstrup, Kristian. 2018. "Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa." Int. J. Mol. Sci. 19, no. 10: 2949.
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