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The Effects of High Fat Diet-Induced Stress on Olfactory Sensitivity, Behaviors, and Transcriptional Profiling in Drosophila melanogaster

Department of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Korea
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Int. J. Mol. Sci. 2018, 19(10), 2855; https://doi.org/10.3390/ijms19102855
Received: 17 July 2018 / Revised: 10 September 2018 / Accepted: 12 September 2018 / Published: 20 September 2018
(This article belongs to the Section Biochemistry)
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Abstract

High-fat diet (HFD) often causes obesity and it has detrimental effects on the sensory system. In particular, sensory-mediated responses are crucial for maintaining energy balance, as they are involved in a metabolic regulation; however, there is still no clear explanation about the relationship between HFD-induced stress and sensory system. To gain insight on how HFD-induced stress affects olfactory sensitivity and behavioral responses, we have used a Drosophila melanogaster model for olfactory and nutrient-related signaling and accessed physiological, behavioral, and transcriptional changes. We demonstrated that lifespan and climbing ability in HFD-treated flies decreased and that olfactory sensitivity and behavioral responses to odorants were changed. Olfactory sensitivity to eight of ten odorants after 14 days on HFD treatment were reduced, while behavioral attraction was increased to benzaldehyde in flies that were treated with HFD. This behavioral and physiological modification in HFD-treated flies for 14 days was accompanied by a significant decrease in DmOrco gene expression in a peripheral olfactory organ, suggesting that is could be involved in the action of metabolic and sensory signal. Gene expression profiles of antennae showed significant differences on the olfactory receptors, odorant-binding proteins, and insulin signaling. Our results suggested that olfactory sensitivity and behavioral responses to HFD-induced stress are mediated through olfactory and nutrient-related signaling pathways. View Full-Text
Keywords: Drosophila melanogaster; high fat diet; olfaction; olfactory receptors; gene expression; climbing behaviors Drosophila melanogaster; high fat diet; olfaction; olfactory receptors; gene expression; climbing behaviors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jung, J.; Kim, D.-I.; Han, G.-Y.; Kwon, H.W. The Effects of High Fat Diet-Induced Stress on Olfactory Sensitivity, Behaviors, and Transcriptional Profiling in Drosophila melanogaster. Int. J. Mol. Sci. 2018, 19, 2855.

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