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Article

Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel?

1
John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
2
IDna Genetics Ltd, Centrum, Norwich Research Park, Norwich NR4 7UG, UK
3
Department of Biology Sciences, University of Auckland, Auckland 1142, New Zealand
4
Department of Crop Physiology, International Centre for Agricultural Research in the Dry Areas (ICARDA), Rabat 10106, Morocco
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Marcello Iriti
Int. J. Mol. Sci. 2017, 18(6), 1205; https://doi.org/10.3390/ijms18061205
Received: 28 April 2017 / Revised: 23 May 2017 / Accepted: 25 May 2017 / Published: 6 June 2017
(This article belongs to the Special Issue Pulses)
One of the traits studied by Mendel in pea (Pisum sativum L.) was the wrinkled-seeded phenotype, and the molecular basis for a mutation underlying this phenotype was discovered in the 1990s. Although the starch-branching enzyme gene mutation identified at the genetic locus r is most likely to be that in seeds available to Mendel in the mid-1800s, it has remained an open question as to whether or not additional natural mutations in this gene exist within Pisum germplasm collections. Here, we explore this question and show that all but two wrinkled-seeded variants in one such collection correspond to either the mutant allele described previously for the r locus or a mutation at a second genetic locus, rb, affecting the gene encoding the large subunit of Adenosine diphosphoglucose (ADP-glucose) pyrophosphorylase; the molecular basis for the rb mutation is described here. The genetic basis for the phenotype of one (JI 2110) of the two lines which are neither r nor rb has been studied in crosses with a round-seeded variant (JI 281); for which extensive genetic marker data were expected. In marked contrast to the trait studied by Mendel and the rb phenotype; the data suggest that the wrinkled-seeded phenotype in JI 2110 is maternally determined, controlled by two genetic loci, and the extent to which it is manifested is very sensitive to the environment. Metabolite analysis of the cotyledons of JI 2110 revealed a profile for sucrose and sucrose-derived compounds that was more similar to that of wild-type round-seeded, than that of wrinkled-seeded r, pea lines. However, the metabolite profile of the seed coat (testa) of JI 2110 was distinct from that of other round-seeded genotypes tested which, together with analysis of recombinant inbred progeny lines, suggests an explanation for the seed phenotype. View Full-Text
Keywords: genetic markers; myoinositol; pea germplasm; r and rb mutations; seed phenotype; seed coat (testa) metabolites; wrinkled seeds genetic markers; myoinositol; pea germplasm; r and rb mutations; seed phenotype; seed coat (testa) metabolites; wrinkled seeds
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MDPI and ACS Style

Rayner, T.; Moreau, C.; Ambrose, M.; Isaac, P.G.; Ellis, N.; Domoney, C. Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel? Int. J. Mol. Sci. 2017, 18, 1205. https://doi.org/10.3390/ijms18061205

AMA Style

Rayner T, Moreau C, Ambrose M, Isaac PG, Ellis N, Domoney C. Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel? International Journal of Molecular Sciences. 2017; 18(6):1205. https://doi.org/10.3390/ijms18061205

Chicago/Turabian Style

Rayner, Tracey, Carol Moreau, Mike Ambrose, Peter G. Isaac, Noel Ellis, and Claire Domoney. 2017. "Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel?" International Journal of Molecular Sciences 18, no. 6: 1205. https://doi.org/10.3390/ijms18061205

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