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Int. J. Mol. Sci. 2017, 18(11), 2467; https://doi.org/10.3390/ijms18112467

Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study

1
Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 21, 80126 Napoli, Italy
2
IRGS, Biogem, Via Camporeale, Ariano Irpino, 83031 Avellino, Italy
3
Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port’Arsa 11, 82100 Benevento, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 14 September 2017 / Revised: 4 November 2017 / Accepted: 7 November 2017 / Published: 20 November 2017
(This article belongs to the Section Molecular Toxicology)
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Abstract

chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus, treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT2 Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose–response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1, Park 2, Sv2b, Gabbr2, Sept5 and Atxn2, were directly related to Parkinson’s onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases. View Full-Text
Keywords: chlorpyrifos; organophosphate insecticides; Mus musculus; developmental neurotoxicity; Parkinson’s disease; neurodegenerative diseases; PCR-array chlorpyrifos; organophosphate insecticides; Mus musculus; developmental neurotoxicity; Parkinson’s disease; neurodegenerative diseases; PCR-array
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Pallotta, M.M.; Ronca, R.; Carotenuto, R.; Porreca, I.; Turano, M.; Ambrosino, C.; Capriglione, T. Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study. Int. J. Mol. Sci. 2017, 18, 2467.

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