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Int. J. Mol. Sci. 2017, 18(1), 181;

The Promoting Effect of the Extracellular Matrix Peptide TNIIIA2 Derived from Tenascin-C in Colon Cancer Cell Infiltration

Department of Gastroenterology, Institute of Clinical Medicine, University of Tsukuba Graduate School, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba 283-8555, Japan
Department of Gastroenterology, Hitachi Medical Education and Research Center, Tsukuba University Hospital, 2-1-1 Jyounancho, Hitachi, Ibaraki 317-0077, Japan
Author to whom correspondence should be addressed.
Academic Editors: Anthony Lemarié and Sylvie Monferran
Received: 16 September 2016 / Revised: 26 December 2016 / Accepted: 11 January 2017 / Published: 17 January 2017
(This article belongs to the Special Issue Integrins in Cancer)
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The extracellular matrix (ECM) molecule tenascin C (TNC) is known to be highly expressed under various pathological conditions such as inflammation and cancer. It has been reported that the expression of TNC is correlated with the malignant potential of cancer. In our laboratory, it was found that the peptide derived from the alternative splicing domain A2 in TNC, termed TNIIIA2, has been shown to influence a variety of cellular processes, such as survival, proliferation, migration, and differentiation. In this study, we investigated the effect of TNC/TNIIIA2 on the invasion and metastasis of colon cancer cells, Colon26-M3.1, or PMF-Ko14, using an in vitro and in vivo experimental system. The degree of cell invasion was increased by the addition of TNC and TNIIIA2 in a dose-dependent manner. The invasion by TNC and TNIIIA2 were suppressed by an MMP inhibitor or TNIIIA2-blocking antibody. In an in vivo experiment, pulmonary metastasis was promoted conspicuously by the addition of TNIIIA2. In this study, we found that colon cancer cell invasion and metastasis was accelerated by TNC/TNIIIA2 via MMP induction. This result suggests the possibility of a new strategy targeting TNC/TNIIIA2 for colon cancer. View Full-Text
Keywords: extracellular matrix; tenascin C; TNIIIA2; matrix metalloproteinase; colon cancer extracellular matrix; tenascin C; TNIIIA2; matrix metalloproteinase; colon cancer

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Suzuki, H.; Sasada, M.; Kamiya, S.; Ito, Y.; Watanabe, H.; Okada, Y.; Ishibashi, K.; Iyoda, T.; Yanaka, A.; Fukai, F. The Promoting Effect of the Extracellular Matrix Peptide TNIIIA2 Derived from Tenascin-C in Colon Cancer Cell Infiltration. Int. J. Mol. Sci. 2017, 18, 181.

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