Next Article in Journal
Infectious Diseases: Pathophysiology, Diagnostics and Prevention
Next Article in Special Issue
Plant-to-Plant Variability in Root Metabolite Profiles of 19 Arabidopsis thaliana Accessions Is Substance-Class-Dependent
Previous Article in Journal
MicroRNA-378 Alleviates Cerebral Ischemic Injury by Negatively Regulating Apoptosis Executioner Caspase-3
Previous Article in Special Issue
Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
Open AccessArticle

Cannibalism Affects Core Metabolic Processes in Helicoverpa armigera Larvae—A 2D NMR Metabolomics Study

by Fredd Vergara 1,*, Amiu Shino 1 and Jun Kikuchi 1,2,3,*
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
Graduate School of Medical Life Science, Yokohama City University, Yokohama 230-0045, Japan
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
Authors to whom correspondence should be addressed.
Academic Editor: Chang Won Choi
Int. J. Mol. Sci. 2016, 17(9), 1470;
Received: 29 April 2016 / Revised: 26 August 2016 / Accepted: 30 August 2016 / Published: 2 September 2016
(This article belongs to the Special Issue Metabolomics in the Plant Sciences)
Cannibalism is known in many insect species, yet its impact on insect metabolism has not been investigated in detail. This study assessed the effects of cannibalism on the metabolism of fourth-instar larvae of the non-predatory insect Helicoverpa armigera (Lepidotera: Noctuidea). Two groups of larvae were analyzed: one group fed with fourth-instar larvae of H. armigera (cannibal), the other group fed with an artificial plant diet. Water-soluble small organic compounds present in the larvae were analyzed using two-dimensional nuclear magnetic resonance (NMR) and principal component analysis (PCA). Cannibalism negatively affected larval growth. PCA of NMR spectra showed that the metabolic profiles of cannibal and herbivore larvae were statistically different with monomeric sugars, fatty acid- and amino acid-related metabolites as the most variable compounds. Quantitation of 1H-13C HSQC (Heteronuclear Single Quantum Coherence) signals revealed that the concentrations of glucose, glucono-1,5-lactone, glycerol phosphate, glutamine, glycine, leucine, isoleucine, lysine, ornithine, proline, threonine and valine were higher in the herbivore larvae. View Full-Text
Keywords: cannibalism; Helicoverpa armigera; HSQC; NMR; metabolomics cannibalism; Helicoverpa armigera; HSQC; NMR; metabolomics
Show Figures

Figure 1

MDPI and ACS Style

Vergara, F.; Shino, A.; Kikuchi, J. Cannibalism Affects Core Metabolic Processes in Helicoverpa armigera Larvae—A 2D NMR Metabolomics Study. Int. J. Mol. Sci. 2016, 17, 1470.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop