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Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea
WCU Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea
The Hormel Institute, University of Minnesota, Austin, MN 55912, USA
Advanced Institutes of Convergence Technology, Seoul National University, Suwon 443-270, Korea
Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong 523808, China
Department of Molecular Medicine, School of Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-799, Korea
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Gopinadhan Paliyath
Int. J. Mol. Sci. 2016, 17(2), 264;
Received: 10 January 2016 / Revised: 13 February 2016 / Accepted: 17 February 2016 / Published: 22 February 2016
(This article belongs to the Special Issue The Mechanism of Action of Food Components in Disease Prevention)
PDF [2125 KB, uploaded 22 February 2016]


Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. In vitro cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC. View Full-Text
Keywords: benzyl isothiocyanate; prostate cancer development; TRAMP; cell cycle arrest benzyl isothiocyanate; prostate cancer development; TRAMP; cell cycle arrest

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Cho, H.J.; Lim, D.Y.; Kwon, G.T.; Kim, J.H.; Huang, Z.; Song, H.; Oh, Y.S.; Kang, Y.-H.; Lee, K.W.; Dong, Z.; Park, J.H.Y. Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest. Int. J. Mol. Sci. 2016, 17, 264.

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