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Int. J. Mol. Sci. 2016, 17(2), 168;

Lingual Epithelial Stem Cells and Organoid Culture of Them

Department of Stem Cell Pathology, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan
Third Department of Internal Medicine, Kansai Medical University, 5-1, 2-chome, Shin-machi, Hirakata-shi, Osaka 573-1010, Japan
Author to whom correspondence should be addressed.
Academic Editor: Miroslav Blumenberg
Received: 23 November 2015 / Revised: 12 January 2016 / Accepted: 18 January 2016 / Published: 28 January 2016
(This article belongs to the Special Issue Molecular Research of Epidermal Stem Cells 2015)
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As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine. View Full-Text
Keywords: lingual epithelium; stem cells; Bmi1; organoid lingual epithelium; stem cells; Bmi1; organoid

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Hisha, H.; Tanaka, T.; Ueno, H. Lingual Epithelial Stem Cells and Organoid Culture of Them. Int. J. Mol. Sci. 2016, 17, 168.

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