Human Intervention Study to Assess the Effects of Supplementation with Olive Leaf Extract on Peripheral Blood Mononuclear Cell Gene Expression
by
Anna Boss 1, Chi Hsiu-Juei Kao 1,2, Pamela M. Murray 2, Gareth Marlow 3, Matthew P. G. Barnett 4 and 
Lynnette R. Ferguson 1,2,*
Anna Boss 1, Chi Hsiu-Juei Kao 1,2, Pamela M. Murray 2, Gareth Marlow 3, Matthew P. G. Barnett 4 and Lynnette R. Ferguson 1,2,*
1
Discipline of Nutrition and Dietetics, The University of Auckland, Auckland 1142, New Zealand
2
Auckland Cancer Research Society, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1142, New Zealand
3
Institute of Medical Genetics, UHW Main Building, Cardiff University, Cardiff CF10 3XQ, UK
4
Food Nutrition & Health Team, Food & Bio-Based Products Group, AgResearch Limited, Palmerston North 4442, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Gopinadhan Paliyath
Int. J. Mol. Sci. 2016, 17(12), 2019; https://doi.org/10.3390/ijms17122019
Received: 12 October 2016 / Revised: 21 November 2016 / Accepted: 23 November 2016 / Published: 2 December 2016
(This article belongs to the Special Issue The Mechanism of Action of Food Components in Disease Prevention)
Olive leaf extract (OLE) has been used for many years for its putative health benefits, but, to date, scientific evidence for the basis of these effects has been weak. Although recent literature has described a link between ailments such as cardiovascular disease, diabetes and cancer and a protective effect of polyphenols in the OLE, the mode of action is still unclear. Here, we describe a double-blinded placebo (PBO)-controlled trial, in which gene expression profiles of peripheral blood mononuclear cells from healthy male volunteers (n = 29) were analysed to identify genes that responded to OLE, following an eight-week intervention with 20 mL daily consumption of either OLE or PBO. Differences between groups were determined using an adjusted linear model. Subsequent analyses indicated downregulation of genes important in inflammatory pathways, lipid metabolism and cancer as a result of OLE consumption. Gene expression was verified by real-time PCR for three genes (EGR1, COX-2 and ID3). The results presented here suggest that OLE consumption may result in health benefits through influencing the expression of genes in inflammatory and metabolic pathways. Future studies with a larger study group, including male and female participants, looking into direct effects of OLE on lipid metabolism and inflammation are warranted.
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Keywords:
polyphenols; transcriptomics; nutraceutical; inflammation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Boss, A.; Kao, C.H.-J.; Murray, P.M.; Marlow, G.; Barnett, M.P.G.; Ferguson, L.R. Human Intervention Study to Assess the Effects of Supplementation with Olive Leaf Extract on Peripheral Blood Mononuclear Cell Gene Expression. Int. J. Mol. Sci. 2016, 17, 2019.
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