Quercetin Improves Postischemic Recovery of Heart Function in Doxorubicin-Treated Rats and Prevents Doxorubicin-Induced Matrix Metalloproteinase-2 Activation and Apoptosis Induction
Abstract
:1. Introduction
2. Results
2.1. Effects of Quercetin on Biometric Parameters
Parameter | C | QCT | DOX | DOX–QCT |
---|---|---|---|---|
BW (g) | 432.7 ± 13.3 | 428.6 ± 21.3 | 384.6 ± 16.8 a | 355.4 ± 17.1 |
Weight gain (g) | 154.8 ± 11.9 | 153.5 ± 14.4 | 107.2 ± 13.2 a | 89.3 ± 16.3 |
HW (g) | 1.43 ± 0.09 | 1.37 ± 0.12 | 1.26 ± 0.07 | 1.11 ± 0.06 |
HW/BW | 3.325 ± 0.196 | 3.162 ± 0.211 | 3.282 ± 0.176 | 3.170 ± 0.174 |
LV (mg) | 870.6 ± 51.3 | 841.7 ± 86.3 | 822.7 ± 39.5 | 740.8 ± 34.0 |
SBP (mm·Hg) | 113 ± 4 | 120 ± 3 | 137 ± 4 a | 118 ± 5 b |
HR (beats/min) | 369 ± 5 | 376 ± 16 | 392 ± 6 a | 368 ± 9 b |
2.2. Electron Microscopic Analysis of Quercetin Effects on Ultrastructural Changes Induced by Doxorubicin
2.3. Quercetin Treatment Modulates the Doxorubicin-Induced Effects on Matrix Metalloproteinase-2
2.4. Quercetin Prevents the Negative Effects of Doxorubicin on Apoptosis Induction and Superoxide Dismutase Inhibition
2.5. Prolonged Application of Quercetin Improves Postischemic Recovery of Cardiac Function of Isolated Rat Hearts
2.6. Cardioprotective Effects of Quercetin Are Associated with Akt Kinase Activation and Connexin-43 Up-Regulation
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Experimental Model
4.3. Systolic Blood Pressure and Heart Rate Measurement
4.4. Perfusion Technique and Determination of Heart Function
4.5. Samples Collection
4.6. Transmission Electron Microscopy
4.7. Preparation of Tissue Protein Fractions and Western Blot Analysis
4.8. Measurement of MMP-2 Activities by Gelatin Zymography
4.9. Determination of Superoxide Dismutase Activity
4.10. Statistical Evaluation
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Barteková, M.; Šimončíková, P.; Fogarassyová, M.; Ivanová, M.; Okruhlicová, Ľ.; Tribulová, N.; Dovinová, I.; Barančík, M. Quercetin Improves Postischemic Recovery of Heart Function in Doxorubicin-Treated Rats and Prevents Doxorubicin-Induced Matrix Metalloproteinase-2 Activation and Apoptosis Induction. Int. J. Mol. Sci. 2015, 16, 8168-8185. https://doi.org/10.3390/ijms16048168
Barteková M, Šimončíková P, Fogarassyová M, Ivanová M, Okruhlicová Ľ, Tribulová N, Dovinová I, Barančík M. Quercetin Improves Postischemic Recovery of Heart Function in Doxorubicin-Treated Rats and Prevents Doxorubicin-Induced Matrix Metalloproteinase-2 Activation and Apoptosis Induction. International Journal of Molecular Sciences. 2015; 16(4):8168-8185. https://doi.org/10.3390/ijms16048168
Chicago/Turabian StyleBarteková, Monika, Petra Šimončíková, Mária Fogarassyová, Monika Ivanová, Ľudmila Okruhlicová, Narcisa Tribulová, Ima Dovinová, and Miroslav Barančík. 2015. "Quercetin Improves Postischemic Recovery of Heart Function in Doxorubicin-Treated Rats and Prevents Doxorubicin-Induced Matrix Metalloproteinase-2 Activation and Apoptosis Induction" International Journal of Molecular Sciences 16, no. 4: 8168-8185. https://doi.org/10.3390/ijms16048168