Next Article in Journal
In Silico Analysis of the Genes Encoding Proteins that Are Involved in the Biosynthesis of the RMS/MAX/D Pathway Revealed New Roles of Strigolactones in Plants
Next Article in Special Issue
Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles
Previous Article in Journal
Limitations and Extensions of the Lock-and-Key Principle: Differences between Gas State, Solution and Solid State Structures
Previous Article in Special Issue
Conformational Analysis of Misfolded Protein Aggregation by FRET and Live-Cell Imaging Techniques
Open AccessReview

Understanding FRET as a Research Tool for Cellular Studies

1
Department of Biophysics and Cell Biology, University of Debrecen, Egyetem tér 1, Nagyerdei Krt. 98, Debrecen 4032, Hungary
2
MTA-DE Cell Biology and Signaling Research Group, Faculty of Medicine, University of Debrecen, Egyetem tér 1, Debrecen 4032, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Herbert Schneckenburger
Int. J. Mol. Sci. 2015, 16(4), 6718-6756; https://doi.org/10.3390/ijms16046718
Received: 27 January 2015 / Accepted: 18 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET) 2015)
Communication of molecular species through dynamic association and/or dissociation at various cellular sites governs biological functions. Understanding these physiological processes require delineation of molecular events occurring at the level of individual complexes in a living cell. Among the few non-invasive approaches with nanometer resolution are methods based on Förster Resonance Energy Transfer (FRET). FRET is effective at a distance of 1–10 nm which is equivalent to the size of macromolecules, thus providing an unprecedented level of detail on molecular interactions. The emergence of fluorescent proteins and SNAP- and CLIP- tag proteins provided FRET with the capability to monitor changes in a molecular complex in real-time making it possible to establish the functional significance of the studied molecules in a native environment. Now, FRET is widely used in biological sciences, including the field of proteomics, signal transduction, diagnostics and drug development to address questions almost unimaginable with biochemical methods and conventional microscopies. However, the underlying physics of FRET often scares biologists. Therefore, in this review, our goal is to introduce FRET to non-physicists in a lucid manner. We will also discuss our contributions to various FRET methodologies based on microscopy and flow cytometry, while describing its application for determining the molecular heterogeneity of the plasma membrane in various cell types. View Full-Text
Keywords: FRET; Methods for measuring FRET; Fluorescence intensity; Fluorescence lifetime; Anisotropy; Major Histocompatibility Complex (MHC); CD1d; IL2; IL15; Immune synapse; ErbB FRET; Methods for measuring FRET; Fluorescence intensity; Fluorescence lifetime; Anisotropy; Major Histocompatibility Complex (MHC); CD1d; IL2; IL15; Immune synapse; ErbB
Show Figures

Figure 1

MDPI and ACS Style

Shrestha, D.; Jenei, A.; Nagy, P.; Vereb, G.; Szöllősi, J. Understanding FRET as a Research Tool for Cellular Studies. Int. J. Mol. Sci. 2015, 16, 6718-6756. https://doi.org/10.3390/ijms16046718

AMA Style

Shrestha D, Jenei A, Nagy P, Vereb G, Szöllősi J. Understanding FRET as a Research Tool for Cellular Studies. International Journal of Molecular Sciences. 2015; 16(4):6718-6756. https://doi.org/10.3390/ijms16046718

Chicago/Turabian Style

Shrestha, Dilip; Jenei, Attila; Nagy, Péter; Vereb, György; Szöllősi, János. 2015. "Understanding FRET as a Research Tool for Cellular Studies" Int. J. Mol. Sci. 16, no. 4: 6718-6756. https://doi.org/10.3390/ijms16046718

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop