MiR-25 Protects Cardiomyocytes against Oxidative Damage by Targeting the Mitochondrial Calcium Uniporter
Abstract
:1. Introduction
2. Results
2.1. MiR-25 Was Remarkably Elevated under Oxidative Stress
2.2. Over-Expression of MiR-25 Protected Cardiomyocytes against Oxidative Damage by Inactivating the Mitochondrial Apoptosis Pathway
2.3. MCU Was a Direct Target of MiR-25
2.4. MiR-25 Significantly Decreased H2O2-Induced Elevation of Mitochondrial Ca2+ Concentration
3. Discussion
4. Methods and Materials
4.1. Cell Culture and Viral Infection
4.2. Quantitative Reverse Transcription-Polymerase Chain Reaction
4.3. Fluorescence-Activated Cell Sorting (FACS)
4.4. Cell Vitality Analysis
4.5. TUNEL Assay
4.6. Immunoblotting
4.7. Luciferase Reporter Assay
4.8. Mitochondrial Calcium Recordings and Analyses
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Share and Cite
Pan, L.; Huang, B.-J.; Ma, X.-E.; Wang, S.-Y.; Feng, J.; Lv, F.; Liu, Y.; Liu, Y.; Li, C.-M.; Liang, D.-D.; et al. MiR-25 Protects Cardiomyocytes against Oxidative Damage by Targeting the Mitochondrial Calcium Uniporter. Int. J. Mol. Sci. 2015, 16, 5420-5433. https://doi.org/10.3390/ijms16035420
Pan L, Huang B-J, Ma X-E, Wang S-Y, Feng J, Lv F, Liu Y, Liu Y, Li C-M, Liang D-D, et al. MiR-25 Protects Cardiomyocytes against Oxidative Damage by Targeting the Mitochondrial Calcium Uniporter. International Journal of Molecular Sciences. 2015; 16(3):5420-5433. https://doi.org/10.3390/ijms16035420
Chicago/Turabian StylePan, Lei, Bi-Jun Huang, Xiu-E Ma, Shi-Yi Wang, Jing Feng, Fei Lv, Yuan Liu, Yi Liu, Chang-Ming Li, Dan-Dan Liang, and et al. 2015. "MiR-25 Protects Cardiomyocytes against Oxidative Damage by Targeting the Mitochondrial Calcium Uniporter" International Journal of Molecular Sciences 16, no. 3: 5420-5433. https://doi.org/10.3390/ijms16035420