Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa
Abstract
:1. Introduction
2. Results and Discussion
Position | δH (J in Hz) | δC, Multiple | 1H–1H COSY | HMBC |
---|---|---|---|---|
1 | 2.80 m | 44.4, CH | H-14α | C-3, -14 |
2 | 2.67–2.78 m | 41.7, CH2 | n.o. c | C-1, -3, -14, -15 |
3 | 208.7, C | |||
4 | 4.34 dd (4.8, 3.6) a | 79.1, CH | OH-4 | C-3, -6 |
5 | 4.33 br d (3.6) a | 76.6, CH | n.o. | C-6 |
6 | 211.5, C | |||
7α | 2.31 d (16.8) | 52.6, CH2 | H-7β | C-6, -8, -9, -18 |
β | 2.44 d (16.8) | H-7α | C-6, -8, -9, -18 | |
8 | 80.2, C | |||
9α | 2.10 br d (14.8) | 42.5, CH2 | H-9β | C-7, -8, -18 |
β | 2.52 dd (14.8, 8.0) | H-9α, H-10 | C-8, -10, -11, -18 | |
10 | 4.67 dd (8.0, 0.8) | 83.3, CH | H-9β, H-11 | C-8, -11, -19 |
11 | 4.49 s | 76.2, CH | H-10, OH-11 | C-9, -10, -13, -19 |
12 | 130.9, C | |||
13 | 6.63 ddd (10.4, 6.8, 0.8) | 147.2 CH | H2-14 | C-11, -19 |
14α | 2.41 ddd (13.6, 6.8, 6.8) | 31.2, CH2 | H-1, H-13, H-14β | C-1, -2, -12, -13, -15 |
β | 3.64 ddd (13.6, 10.4, 3.6) b | H-13, H-14α | C-2 | |
15 | 147.0, C | |||
16a | 4.87 d (0.8) | 110.4, CH2 | H-16b, H3-17 | C-1, -15, -17 |
b | 4.89 d (0.8) | H-16a, H3-17 | C-1, -15, -17 | |
17 | 1.79 s | 20.8, CH3 | H2-16 | C-1, -15, -16 |
18 | 1.42 s | 25.6, CH3 | C-7, -8, -9 | |
19 | 169.2, C | |||
OH-4 | 3.66 d (4.8) b | H-4 | n.o. | |
OH-11 | 2.01 br s | H-11 | n.o. |
Compounds | Cell Lines IC50 (μg/mL) | ||||||
---|---|---|---|---|---|---|---|
CCRF-CEM | HL-60 | K-562 | U-937 | DLD-1 | LNCaP | MCF7 | |
1 | 11.07 | 11.11 | NA | NA | NA | NA | NA |
2 | 4.21 | 10.38 | 18.07 | 10.08 | NA | 15.33 | NA |
Doxorubicin a | 0.01 | 0.01 | 0.35 | 0.05 | 0.49 | 0.10 | 0.16 |
3. Experimental Section
3.1. General Experimental Procedures
3.2. Animal Material
3.3. Extraction and Isolation
3.4. Molecular Mechasnics Calculations
3.5. MTT Antiproliferative Assay
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chen, W.-F.; Yin, C.-T.; Cheng, C.-H.; Lu, M.-C.; Fang, L.-S.; Wang, W.-H.; Wen, Z.-H.; Chen, J.-J.; Wu, Y.-C.; Sung, P.-J. Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa. Int. J. Mol. Sci. 2015, 16, 3298-3306. https://doi.org/10.3390/ijms16023298
Chen W-F, Yin C-T, Cheng C-H, Lu M-C, Fang L-S, Wang W-H, Wen Z-H, Chen J-J, Wu Y-C, Sung P-J. Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa. International Journal of Molecular Sciences. 2015; 16(2):3298-3306. https://doi.org/10.3390/ijms16023298
Chicago/Turabian StyleChen, Wu-Fu, Chen-Ting Yin, Ching-Hsiao Cheng, Mei-Chin Lu, Lee-Shing Fang, Wei-Hsien Wang, Zhi-Hong Wen, Jih-Jung Chen, Yang-Chang Wu, and Ping-Jyun Sung. 2015. "Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa" International Journal of Molecular Sciences 16, no. 2: 3298-3306. https://doi.org/10.3390/ijms16023298