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Int. J. Mol. Sci. 2015, 16(2), 3178-3187;

CXCL13 Promotes the Effect of Bone Marrow Mesenchymal Stem Cells (MSCs) on Tendon-Bone Healing in Rats and in C3HIOT1/2 Cells

* ,
Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 13 November 2014 / Revised: 14 January 2015 / Accepted: 21 January 2015 / Published: 30 January 2015
(This article belongs to the Section Biochemistry)
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Objectives: Mesenchymal stem cells (MSCs) are potential effective therapy for tissue repair and bone regeneration. In present study, the effects of CXC chemokine ligand-13 (CXCL13) were evaluated on tendon-bone healing of rats. Methods: Tendon bone healing of the rat model was established and biomechanical testing was performed at 2, 4, 8 weeks after surgery. Murine mesenchymal cell line (C3HIOT1/2 cells) was cultured. The expression of miRNA-23a was detected by real-time PCR. The protein expression of ERK1/2, JNK and p38 was detected by western blotting. MiR-23a mimic and inhibitor were used to overexpress or silence the expression of miR-23a. Results: MSCs significantly elevated the levels of ultimate load to failure, stiffness and stress in specimens of rats, the effects of which were enhanced by CXCL13. The expression of miR-23a was down-regulated and the protein of ERK1/2 level was up-regulated by CXCL13 treatment in both in vivo and in vitro experiments. ERK1/2 expression was elevated by overexpression of miR-23a and reduced by miR-23a inhibitor. Conclusions: These findings revealed that CXCL13 promoted the tendon-bone healing in rats with MSCs treatment, and implied that the activation of ERK1/2 via miR-23a was involved in the process of MSCs treated bone regeneration. View Full-Text
Keywords: marrow mesenchymal stem cells; rat; tendon-bone healing; CXCL13 marrow mesenchymal stem cells; rat; tendon-bone healing; CXCL13

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Tian, F.; Ji, X.-L.; Xiao, W.-A.; Wang, B.; Wang, F. CXCL13 Promotes the Effect of Bone Marrow Mesenchymal Stem Cells (MSCs) on Tendon-Bone Healing in Rats and in C3HIOT1/2 Cells. Int. J. Mol. Sci. 2015, 16, 3178-3187.

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