Next Article in Journal
Melatonin Stimulates Dendrite Formation and Complexity in the Hilar Zone of the Rat Hippocampus: Participation of the Ca++/Calmodulin Complex
Previous Article in Journal
Surveillance and Cleavage of Eukaryotic tRNAs
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(1), 1894-1906;

Isolation and Expression of NAC Genes during Persimmon Fruit Postharvest Astringency Removal

Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
College of Food Science and Engineering, Wuhan Polytechnic University, Changqing Campus, Wuhan 430023, China
Plant & Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 9 December 2014 / Accepted: 9 January 2015 / Published: 15 January 2015
Full-Text   |   PDF [2232 KB, uploaded 15 January 2015]   |  


NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of “Mopan” persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed. View Full-Text
Keywords: persimmon; deastringency; NAC; low oxygen stress; transcription factor persimmon; deastringency; NAC; low oxygen stress; transcription factor

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Min, T.; Wang, M.-M.; Wang, H.; Liu, X.; Fang, F.; Grierson, D.; Yin, X.-R.; Chen, K.-S. Isolation and Expression of NAC Genes during Persimmon Fruit Postharvest Astringency Removal. Int. J. Mol. Sci. 2015, 16, 1894-1906.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top