Triethanolamine Stabilization of Methotrexate-β-Cyclodextrin Interactions in Ternary Complexes
AbstractThe interaction of methotrexate (MTX) with beta-cyclodextrin (β-CD) in the presence of triethanolamine (TEA) was investigated with the aim to elucidate the mechanism whereby self-assembly cyclodextrin systems work in association with this third component. Solubility diagram studies showed synergic increment of the MTX solubility to be about thirty-fold. Experiments using 2D ROESY and molecular modeling studies revealed the inclusion of aromatic ring III of the drug into β-CD cavity, in which TEA contributes by intensifying MTX interaction with β-CD and stabilizes MTX:β-CD:TEA ternary complex by electrostatic interaction. The maintenance of these interactions in solid phase was also studied in ternary MTX:β-CD:TEA and comparisons were made with freeze dried binary MTX:β-CD and physical mixtures. FTIR studies evidenced that MTX–β-CD interaction remained in solid ternary complexes, which was also supported by thermal (differential scanning calorimetry (DSC), thermogravimetric analysis (TG)/first derivative of TG analysis (DTG) and C,N,H elementary analysis) and structural (X-ray diffraction analysis, (XRD)) studies, mainly regarding the increment of drug stability. The efficient in vitro drug dissolution studies successfully demonstrated the contribution of ternary complexes, which highlights the importance of this possible new raw material for further applications in drug delivery systems. View Full-Text
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Barbosa, J.A.A.; Zoppi, A.; Quevedo, M.A.; de Melo, P.N.; de Medeiros, A.S.A.; Streck, L.; de Oliveira, A.R.; Fernandes-Pedrosa, M.F.; Longhi, M.R.; da Silva-Júnior, A.A. Triethanolamine Stabilization of Methotrexate-β-Cyclodextrin Interactions in Ternary Complexes. Int. J. Mol. Sci. 2014, 15, 17077-17099.
Barbosa JAA, Zoppi A, Quevedo MA, de Melo PN, de Medeiros ASA, Streck L, de Oliveira AR, Fernandes-Pedrosa MF, Longhi MR, da Silva-Júnior AA. Triethanolamine Stabilization of Methotrexate-β-Cyclodextrin Interactions in Ternary Complexes. International Journal of Molecular Sciences. 2014; 15(9):17077-17099.Chicago/Turabian Style
Barbosa, Jahamunna A.A.; Zoppi, Ariana; Quevedo, Mario A.; de Melo, Polyanne N.; de Medeiros, Arthur S.A.; Streck, Letícia; de Oliveira, Alice R.; Fernandes-Pedrosa, Matheus F.; Longhi, Marcela R.; da Silva-Júnior, Arnóbio A. 2014. "Triethanolamine Stabilization of Methotrexate-β-Cyclodextrin Interactions in Ternary Complexes." Int. J. Mol. Sci. 15, no. 9: 17077-17099.