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Open AccessArticle

Discovery and Characterization of Two Novel Salt-Tolerance Genes in Puccinellia tenuiflora

Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China
Asian Natural Environmental Science Center, University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(9), 16469-16483;
Received: 22 July 2014 / Revised: 25 August 2014 / Accepted: 5 September 2014 / Published: 18 September 2014
(This article belongs to the Section Biochemistry)
Puccinellia tenuiflora is a monocotyledonous halophyte that is able to survive in extreme saline soil environments at an alkaline pH range of 9–10. In this study, we transformed full-length cDNAs of P. tenuiflora into Saccharomyces cerevisiae by using the full-length cDNA over-expressing gene-hunting system to identify novel salt-tolerance genes. In all, 32 yeast clones overexpressing P. tenuiflora cDNA were obtained by screening under NaCl stress conditions; of these, 31 clones showed stronger tolerance to NaCl and were amplified using polymerase chain reaction (PCR) and sequenced. Four novel genes encoding proteins with unknown function were identified; these genes had no homology with genes from higher plants. Of the four isolated genes, two that encoded proteins with two transmembrane domains showed the strongest resistance to 1.3 M NaCl. RT-PCR and northern blot analysis of P. tenuiflora cultured cells confirmed the endogenous NaCl-induced expression of the two proteins. Both of the proteins conferred better tolerance in yeasts to high salt, alkaline and osmotic conditions, some heavy metals and H2O2 stress. Thus, we inferred that the two novel proteins might alleviate oxidative and other stresses in P. tenuiflora. View Full-Text
Keywords: salt stress; halophyte; Puccinellia tenuiflora; salt-tolerant FOX-yeast lines salt stress; halophyte; Puccinellia tenuiflora; salt-tolerant FOX-yeast lines
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Li, Y.; Takano, T.; Liu, S. Discovery and Characterization of Two Novel Salt-Tolerance Genes in Puccinellia tenuiflora. Int. J. Mol. Sci. 2014, 15, 16469-16483.

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