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Open AccessArticle

Enamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralization

Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Blindern, N-0317 Oslo, Norway
Department of Medical Biochemistry, Oslo University Hospital, N-0450 Oslo, Norway
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(5), 7731-7749;
Received: 26 February 2014 / Revised: 26 March 2014 / Accepted: 14 April 2014 / Published: 5 May 2014
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells 2014)
Enamel matrix derivative (EMD) has been found to induce reactive dentin formation; however the molecular mechanisms involved are unclear. The effect of EMD (5–50 μg/mL) on primary human pulp cells were compared to untreated cells and cells incubated with 10−8 M dexamethasone (DEX) for 1, 2, 3, 7, and 14 days in culture. Expression analysis using Affymetrix microchips demonstrated that 10 μg/mL EMD regulated several hundred genes and stimulated the gene expression of proteins involved in mesenchymal proliferation and differentiation. Both EMD and DEX enhanced the expression of amelogenin (amel), and the dentinogenic markers dentin sialophosphoprotein (DSSP) and dentin matrix acidic phosphoprotein 1 (DMP1), as well as the osteogenic markers osteocalcin (OC, BGLAP) and collagen type 1 (COL1A1). Whereas, only EMD had effect on alkaline phosphatase (ALP) mRNA expression, the stimulatory effect were verified by enhanced secretion of OC and COL1A from EMD treated cells, and increased ALP activity in cell culture medium after EMD treatment. Increased levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant proteins (MCP-1) in the cell culture medium were also found. Consequently, the suggested effect of EMD is to promote differentiation of pulp cells and increases the potential for pulpal mineralization to favor reactive dentine formation. View Full-Text
Keywords: pulp cells; enamel matrix derivative (EMD); bone; dentin; epigenetic factors pulp cells; enamel matrix derivative (EMD); bone; dentin; epigenetic factors
MDPI and ACS Style

Riksen, E.A.; Landin, M.A.; Reppe, S.; Nakamura, Y.; Lyngstadaas, S.P.; Reseland, J.E. Enamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralization. Int. J. Mol. Sci. 2014, 15, 7731-7749.

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