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Int. J. Mol. Sci. 2014, 15(12), 21896-21912;

Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China
Author to whom correspondence should be addressed.
Received: 21 October 2014 / Revised: 22 November 2014 / Accepted: 24 November 2014 / Published: 28 November 2014
(This article belongs to the Section Biochemistry)
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We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy. View Full-Text
Keywords: bacterial artificial chromosome; wheat; descending pool bacterial artificial chromosome; wheat; descending pool

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Cao, W.; Fu, B.; Wu, K.; Li, N.; Zhou, Y.; Gao, Z.; Lin, M.; Li, G.; Wu, X.; Ma, Z.; Jia, H. Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries. Int. J. Mol. Sci. 2014, 15, 21896-21912.

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