Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression
Abstract
:1. Introduction
2. Results
2.1. MTT Analysis and Caspase-3 Activity Measurement
2.2. Flow-Cytometric Analysis for Detecting Cellular Apoptosis
2.3. Inhibition of Catalpol on MMP-2
2.4. Catalpol Activates miR-200 Expression
2.5. Overexpression of miR-200 and MMP-2 Expression
2.6. Anti-miR-200 Can Reverse the Effect of Catalpol
3. Discussion
4. Experimental Section
4.1. Chemicals and Reagents
4.2. Cell Culture
4.3. Cell Viability Assay
4.4. Caspase-3 Activity Assays
4.5. Flow Cytometry
4.6. Gelatin Zymography Assays of MMP-2
4.7. Q-PCR Analysis of miR-200 Expression
4.8. Transfection of miR-200 and Anti-miR-200
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gao, N.; Tian, J.-X.; Shang, Y.-H.; Zhao, D.-Y.; Wu, T. Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression. Int. J. Mol. Sci. 2014, 15, 19394-19405. https://doi.org/10.3390/ijms151119394
Gao N, Tian J-X, Shang Y-H, Zhao D-Y, Wu T. Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression. International Journal of Molecular Sciences. 2014; 15(11):19394-19405. https://doi.org/10.3390/ijms151119394
Chicago/Turabian StyleGao, Na, Jian-Xin Tian, Yu-Hong Shang, Dan-Yi Zhao, and Tao Wu. 2014. "Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression" International Journal of Molecular Sciences 15, no. 11: 19394-19405. https://doi.org/10.3390/ijms151119394