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Open AccessArticle

Changing Microspatial Patterns of Sulfate-Reducing Microorganisms (SRM) during Cycling of Marine Stromatolite Mats

1
Department of Urban and Landscape Planning, School of Urban Planning, "Ion Mincu" University of Architecture and Urban Planning, str. Academiei nr. 18-20, sector 1, Bucharest 010014, Romania
2
National Institute for Research and Development in Constructions, Urbanism and Sustainable Spatial Development URBAN-INCERC, sos. Pantelimon, nr. 266, sector 2, Bucharest 021652, Romania
3
Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC 29208, USA
4
Center for Integrative GeoSciences, University of Connecticut, 345 Mansfield Rd., U-2045 Storrs, CT 06269, USA
*
Author to whom correspondence should be addressed.
Present address: Department of Chemistry, University Duisburg-Essen, Universit
Int. J. Mol. Sci. 2014, 15(1), 850-877; https://doi.org/10.3390/ijms15010850
Received: 1 November 2013 / Revised: 20 December 2013 / Accepted: 30 December 2013 / Published: 9 January 2014
(This article belongs to the Special Issue Biofilms: Extracellular Bastions of Bacteria)
Microspatial arrangements of sulfate-reducing microorganisms (SRM) in surface microbial mats (~1.5 mm) forming open marine stromatolites were investigated. Previous research revealed three different mat types associated with these stromatolites, each with a unique petrographic signature. Here we focused on comparing “non-lithifying” (Type-1) and “lithifying” (Type-2) mats. Our results revealed three major trends: (1) Molecular typing using the dsrA probe revealed a shift in the SRM community composition between Type-1 and Type-2 mats. Fluorescence in-situ hybridization (FISH) coupled to confocal scanning-laser microscopy (CSLM)-based image analyses, and 35SO42−-silver foil patterns showed that SRM were present in surfaces of both mat types, but in significantly (p < 0.05) higher abundances in Type-2 mats. Over 85% of SRM cells in the top 0.5 mm of Type-2 mats were contained in a dense 130 µm thick horizontal layer comprised of clusters of varying sizes; (2) Microspatial mapping revealed that locations of SRM and CaCO3 precipitation were significantly correlated (p < 0.05); (3) Extracts from Type-2 mats contained acylhomoserine-lactones (C4- ,C6- ,oxo-C6,C7- ,C8- ,C10- ,C12- , C14-AHLs) involved in cell-cell communication. Similar AHLs were produced by SRM mat-isolates. These trends suggest that development of a microspatially-organized SRM community is closely-associated with the hallmark transition of stromatolite surface mats from a non-lithifying to a lithifying state. View Full-Text
Keywords: biofilms; EPS; microbial mats; microspatial; sulfate-reducing microorganisms; dsrA probe; chemical signals; CaCO3; AHLs; 35SO42− silver-foil biofilms; EPS; microbial mats; microspatial; sulfate-reducing microorganisms; dsrA probe; chemical signals; CaCO3; AHLs; 35SO42− silver-foil
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MDPI and ACS Style

Petrisor, A.I.; Szyjka, S.; Kawaguchi, T.; Visscher, P.T.; Norman, R.S.; Decho, A.W. Changing Microspatial Patterns of Sulfate-Reducing Microorganisms (SRM) during Cycling of Marine Stromatolite Mats. Int. J. Mol. Sci. 2014, 15, 850-877. https://doi.org/10.3390/ijms15010850

AMA Style

Petrisor AI, Szyjka S, Kawaguchi T, Visscher PT, Norman RS, Decho AW. Changing Microspatial Patterns of Sulfate-Reducing Microorganisms (SRM) during Cycling of Marine Stromatolite Mats. International Journal of Molecular Sciences. 2014; 15(1):850-877. https://doi.org/10.3390/ijms15010850

Chicago/Turabian Style

Petrisor, Alexandru I.; Szyjka, Sandra; Kawaguchi, Tomohiro; Visscher, Pieter T.; Norman, Robert S.; Decho, Alan W. 2014. "Changing Microspatial Patterns of Sulfate-Reducing Microorganisms (SRM) during Cycling of Marine Stromatolite Mats" Int. J. Mol. Sci. 15, no. 1: 850-877. https://doi.org/10.3390/ijms15010850

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