Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin
Abstract
:1. Introduction
2. Results and Discussions
2.1. Design of the MSP
2.2. Synchronization and Computer Control
2.3. PYP Spectra
2.4. α-Phycoerythrocyanin (α-PEC) Spectra
2.5. Performance and Outlook
3. Experimental Section
4. Conclusions
Acknowledgements
Conflicts of Interest
References
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Solution | Crystal | ||||||
---|---|---|---|---|---|---|---|
Temperature | 295 K | 273 K | 303 K | ||||
Macroscopic rates Λi (s−1) This work | 3.4 × 103 | 2.2 | 3.8 × 102 | 16.4 | 1.4 | 4.3 × 102 | 25 |
Relaxation times τi (ms) This work | 0.29 | 450 | 2.6 | 61 | 700 | 2.3 | 40 |
Relaxation times from 5D-crystallography (ms) [15] | n.a. | n.a. | 0.7 | 73 | n.o. | 6 | n.o. |
Relaxation times from TR-spectroscopy (ms), 293 K [38] | 0.26 | 360 | n.d. | n.d. | n.d. | 4 | 20 |
Sample | Spectrometer | |
---|---|---|
Xenon | 500 μW | 50 μW |
Laser (per pulse) | 2 mJ | n. a. |
a 380 μJ | n. a. | |
LED | 36 mW | n. a. |
1 μs | 250 ns | 10 ns | |
---|---|---|---|
total number of photons | 1.7 × 108 | 4.3 × 107 | 1.7 × 106 |
photons/channel, 400 μm slit | 1.7 × 105 | 4.1 × 104 | 1660 |
photons/channel, 50 μm slit | 2 × 104 | 5 × 103 | 200 |
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Purwar, N.; Tenboer, J.; Tripathi, S.; Schmidt, M. Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin. Int. J. Mol. Sci. 2013, 14, 18881-18898. https://doi.org/10.3390/ijms140918881
Purwar N, Tenboer J, Tripathi S, Schmidt M. Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin. International Journal of Molecular Sciences. 2013; 14(9):18881-18898. https://doi.org/10.3390/ijms140918881
Chicago/Turabian StylePurwar, Namrta, Jason Tenboer, Shailesh Tripathi, and Marius Schmidt. 2013. "Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin" International Journal of Molecular Sciences 14, no. 9: 18881-18898. https://doi.org/10.3390/ijms140918881