Next Article in Journal
Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae
Previous Article in Journal
Nanoparticle-Based Systems for T1-Weighted Magnetic Resonance Imaging Contrast Agents
Open AccessArticle

Effect of Plant Derived Antimicrobials on Salmonella Enteritidis Adhesion to and Invasion of Primary Chicken Oviduct Epithelial Cells in vitro and Virulence Gene Expression

Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(5), 10608-10625; https://doi.org/10.3390/ijms140510608
Received: 18 February 2013 / Revised: 23 April 2013 / Accepted: 1 May 2013 / Published: 21 May 2013
(This article belongs to the Section Biochemistry)
Salmonella Enteritidis (SE) is a major foodborne pathogen in the United States and one of the most frequently reported Salmonella serotypes globally. Eggs are the most common food product associated with SE infections in humans. The pathogen colonizes the intestinal tract in layers, and migrates to reproductive organs systemically. Since adhesion to and invasion of chicken oviduct epithelial cells (COEC) is critical for SE colonization in reproductive tract, reducing these virulence factors could potentially decrease egg yolk contamination. This study investigated the efficacy of sub-inhibitory concentrations of three plant-derived antimicrobials (PDAs), namely carvacrol, thymol and eugenol in reducing SE adhesion to and invasion of COEC, and survival in chicken macrophages. In addition, the effect of PDAs on SE genes critical for oviduct colonization and macrophage survival was determined using real-time quantitative PCR (RT-qPCR). All PDAs significantly reduced SE adhesion to and invasion of COEC (p < 0.001). The PDAs, except thymol consistently decreased SE survival in macrophages (p < 0.001). RT-qPCR results revealed down-regulation in the expression of genes involved in SE colonization and macrophage survival (p < 0.001). The results indicate that PDAs could potentially be used to control SE colonization in chicken reproductive tract; however, in vivo studies validating these results are warranted. View Full-Text
Keywords: Salmonella; plant-derived antimicrobials; oviduct; macrophages; gene expression Salmonella; plant-derived antimicrobials; oviduct; macrophages; gene expression
Show Figures

MDPI and ACS Style

Upadhyaya, I.; Upadhyay, A.; Kollanoor-Johny, A.; Darre, M.J.; Venkitanarayanan, K. Effect of Plant Derived Antimicrobials on Salmonella Enteritidis Adhesion to and Invasion of Primary Chicken Oviduct Epithelial Cells in vitro and Virulence Gene Expression. Int. J. Mol. Sci. 2013, 14, 10608-10625. https://doi.org/10.3390/ijms140510608

AMA Style

Upadhyaya I, Upadhyay A, Kollanoor-Johny A, Darre MJ, Venkitanarayanan K. Effect of Plant Derived Antimicrobials on Salmonella Enteritidis Adhesion to and Invasion of Primary Chicken Oviduct Epithelial Cells in vitro and Virulence Gene Expression. International Journal of Molecular Sciences. 2013; 14(5):10608-10625. https://doi.org/10.3390/ijms140510608

Chicago/Turabian Style

Upadhyaya, Indu; Upadhyay, Abhinav; Kollanoor-Johny, Anup; Darre, Michael J.; Venkitanarayanan, Kumar. 2013. "Effect of Plant Derived Antimicrobials on Salmonella Enteritidis Adhesion to and Invasion of Primary Chicken Oviduct Epithelial Cells in vitro and Virulence Gene Expression" Int. J. Mol. Sci. 14, no. 5: 10608-10625. https://doi.org/10.3390/ijms140510608

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop