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Evidence for the Involvement of RhoA Signaling in the Ethanol-Induced Increase in Intestinal Epithelial Barrier Permeability

Department of Gastroenterology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang 110001, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(2), 3946-3960;
Received: 4 January 2013 / Revised: 19 January 2013 / Accepted: 24 January 2013 / Published: 18 February 2013
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells)
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In this work, we investigated the potential role of the small G protein RhoA in ethanol-induced tight junction (TJ) protein disassembly and increased intestinal epithelial barrier (IEB) permeability. Our study used Caco-2 cells as an in vitro IEB model and RhoA short hairpin RNA (shRNA) interference to establish whether RhoA plays a role in ethanol-induced TJ opening. RhoA shRNA interference partially inhibited epithelial leakage and restored normal transepithelial electrical resistance (TEER) values in the IEB. Moreover, RhoA shRNA interference prevented a shift in occludin distribution from insoluble to soluble fractions. Additionally, RhoA shRNA interference inhibited the ethanol-induced expression of zonula occludens-1 (ZO-1). Finally, RhoA shRNA interference inhibited an ethanol-induced increase in RhoA activity. The contributions of RhoA to an ethanol-induced increase in IEB permeability are associated with TJ disassembly. View Full-Text
Keywords: RhoA; ethanol; IEB; tight junction RhoA; ethanol; IEB; tight junction

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Tong, J.; Wang, Y.; Chang, B.; Zhang, D.; Wang, B. Evidence for the Involvement of RhoA Signaling in the Ethanol-Induced Increase in Intestinal Epithelial Barrier Permeability. Int. J. Mol. Sci. 2013, 14, 3946-3960.

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